Discover insights from thousands of peer-reviewed papers on microbial electrochemical systems
Shuai Lou, Xinbai Jiang, Dan Chen et al.
RSC Advances · 2015
In this study, a membrane-free bio-contact coupled bioelectrochemical system (BC-BES) was established for the enhanced reductive transformation of p -nitrophenol (PNP).
Han Li, Ying Cui, Fei Wang et al.
PLOS ONE · 2023
In this experiment, we took reflux sludge, sludge from an aeration tank, and soil from roots as microbial inoculating sources for an electrochemical device for denitrification with high-throughput sequencing on cathodic biofilms. The efficiency of nitrate nitrogen removal using different microbial inoculates varied among voltages. The optimal voltages for denitrification of reflux sludge, aeration tank sludge, and root soil were 0.7V, 0.5V, and 0.5V, respectively. Further analysis revealed that the respective voltages had a significant effect upon microbial growth from the respective inoculates. Proteobacteria and Firmicutes were the main denitrifying microbes. With the addition of low current (produced by the applied voltage), the Chao1, Shannon and Simpson indexes of the diversity of microorganisms in soil inoculation sources increased, indicating that low current can increase the diversity and richness of the microorganisms, while the reflux sludge and aeration tank sludge showed different changes. Low-current stimulation decreased microbial diversity to a certain extent. Pseudomonas showed a trend of decline with increasing applied voltage, in which the MEC (microbial electrolysis cell) of rhizosphere soil as inoculates decreased most significantly from 77.05% to 12.58%, while the MEC of Fusibacter showed a significant increase, and the sludge of reflux sludge, aeration tank and rhizosphere soil increased by 31.12%, 18.7% and 34.6%, respectively. The applied voltage also significantly increased the abundance of Azoarcus in communities from the respective inoculates.
Yimeng Feng, Xuya Zhu, Xiulin Huang et al.
Energies · 2025
Because of their high persistence, polycyclic aromatic hydrocarbons (PAHs) are found in a wide range of settings and pose a health risk to both humans and other organisms. Degradation of PAHs is an essential part of environmental management. By combining biological metabolism and electrochemical processes, bioelectrochemical systems (BESs) can degrade PAHs and provide important applications by converting the chemical energy of pollutants into electrical energy for energy conversion and recovery. This review provides a comprehensive introduction to PAH degradation by BESs, including PAH sources, degradation effects of BESs, performance enhancement methods, degradation pathways, and dominant microorganisms. By focusing on the relevant research in recent years, the main innovative research focuses on the optimization of the configuration, the electrode preparation, and the media additions to improve the removal performance of PAHs. It demonstrates the potential of BESs in the field of environmental remediation, especially their effectiveness in treating difficult-to-degrade pollutants such as PAHs, by concentrating on the application and mechanism of BESs in PAH degradation. This review is intended to provide the inexperienced reader with an insight into this research area and to point out directions for future research, especially in the design optimization of BESs and microbial community analysis.
Nesrine Saidi, Benjamin Erable, Luc Etchevery et al.
Frontiers in Microbiology · 2025
Thiabendazole (TBZ), a recalcitrant fungicide, is frequently applied in postharvest fruit treatment and generates significant volumes of industrial wastewater (WW) that conventional treatment plants cannot handle. This explores a bioelectrochemical system (BES) for TBZ degradation using Tunisian hypersaline sediments (THSs) as inoculum. Four sets of BES, along with biological controls, were tested using THS subjected to different levels of TBZ biostimulation. Sediments underwent one, two, or three biostimulation phases with increasing TBZ concentrations (0, 10, 100, and 300 mg kg −1 ). Potentiostatic control was applied to BES, polarized at 0.1 V vs. saturated calomel reference electrode (SCE), with a carbon felt working electrode (72 cm 2 L −1 ) and maintained at 25°C. While current production was very low, sediments biostimulated with 100 mg kg −1 kg TBZ produced the highest current density (3.2 mA m −2 ), a 5-fold increase over untreated sediments (0.6 mA m −2 ). GC-FID analysis showed >99% TBZ degradation in all reactors. The TBZ half-elimination time from 27 days with biological treatments to 19 days in BES and further to 6 days following biostimulation. Bacterial analysis revealed a substantial microbial community shift after biostimulation, with a reduction in Bacillota (−64%) and an increase in Proteobacteria (+62%), dominated by Pseudomonas (45%) and Marinobacter (16%). These findings provide insight into the selective potential of biostimulation cycles to enhance microbial community composition and improve BES performance for TBZ wastewater treatment.
Shixiang Dai, F. Harnisch, Mohammad Sufian Bin-Hudari et al.
Microbial Biotechnology · 2022
Treatment of wastewater contaminated with high sulfate concentrations is an environmental imperative lacking a sustainable and environmental friendly technological solution. Microbial electrochemical technology (MET) represents a promising approach for sulfate reduction. In MET, a cathode is introduced as inexhaustible electron source for promoting sulfate reduction via direct or mediated electron transfer. So far, this is mainly studied in batch mode representing straightforward and easy‐to‐use systems, but their practical implementation seems unlikely, as treatment capacities are limited. Here, we investigated bioelectrochemical sulfate reduction in flow mode and achieved removal efficiencies (Esulfate, 89.2 ± 0.4%) being comparable to batch experiments, while sulfate removal rates (Rsulfate, 3.1 ± 0.2 mmol L−1) and Coulombic efficiencies (CE, 85.2 ± 17.7%) were significantly increased. Different temperatures and hydraulic retention times (HRT) were applied and the best performance was achieved at HRT 3.5 days and 30°C. Microbial community analysis based on amplicon sequencing demonstrated that sulfate reduction was mainly performed by prokaryotes belonging to the genera Desulfomicrobium, Desulfovibrio, and Desulfococcus, indicating that hydrogenotrophic and heterotrophic sulfate reduction occurred by utilizing cathodically produced H2 or acetate produced by homoacetogens (Acetobacterium). The advantage of flow operation for bioelectrochemical sulfate reduction is likely based on higher absolute biomass, stable pH, and selection of sulfate reducers with a higher sulfide tolerance, and improved ratio between sulfate‐reducing prokaryotes and homoacetogens.
Yiying Yan, Qiang Wang, Liping Huang et al.
Journal of Chemical Technology & Biotechnology · 2024
Electrochemically active bacteria (EAB) capable of bidirectional extracellular electron transfer (EET), either outward or inward EET, largely control the efficiency of interactions and electrical communication between biofilm and electrode and, thus, control the performance of bioelectrochemical systems (BESs) for heavy metals removal. However, the behavior of such metallurgical EAB capable of bidirectional EET has yet to be investigated, and the role of extracellular polymeric substances (EPS) in these switchable EAB with bidirectional EET and in the presence of heavy metals remains unexplored in the single‐chamber BESs treating heavy metal‐based wastewaters that are limited by carbon/electron sources or electron acceptors.The biofilms of the Cr(VI)‐tolerant EAB Stenotrophomonas sp. YS1 and Serratia marcescens Q1 exhibited bidirectional EET metabolizing either organic (acetate) or inorganic (HCO3−) species with simultaneous removal of Cr(VI) in single‐chamber BESs. Q1 inward EET uptake of electrons was more efficient than that of YS1 (165 μA vs. 118 μA); meanwhile, YS1 outward EET was more efficient than Q1 (8.0 μA vs. 4.7–5.2 μA). The adaptive electrochemically‐tunable EPS in both biofilm strains was regulated by the direction of the EET (inward or outward) in the presence of Cr(VI) and circuital current.This study demonstrates the switching properties of EAB, such as Stenotrophomonas sp. or S. marcescens, that are capable of bidirectional EET to or from the electrodes, and it displays the regulation of such responses with the amount and compositional diversity of the biofilms’ EPS, giving a comprehensive appreciation of tunable EPS for Cr(VI)‐wastewater treatment in single‐chamber BESs. © 2024 Society of Chemical Industry (SCI).
Yan Li, Zhiheng Xu, Dingyi Cai et al.
Environmental Science: Water Research & Technology · 2016
The slow growth rate of anammox bacteria is a pressing problem for system efficiency and stability.
Yuting He, Qian Fu, Jun Li et al.
Small · 2024
Abstract The utility of electrochemical active biofilm in bioelectrochemical systems has received considerable attention for harvesting energy and chemical products. However, the slow electron transfer between biofilms and electrodes hinders the enhancement of performance and still remains challenging. Here, using Fe 3 O 4 /L‐Cys nanoparticles as precursors to induce biomineralization, a facile strategy for the construction of an effective electron transfer pathway through biofilm and biological/inorganic interface is proposed, and the underlying mechanisms are elucidated. Taking advantage of an on‐chip interdigitated microelectrode array (IDA), the conductive current of biofilm that is related to the electron transfer process within biofilm is characterized, and a 2.10‐fold increase in current output is detected. The modification of Fe 3 O 4 /L‐Cys on the electrode surface facilitates the electron transfer between the biofilm and the electrode, as the bio/inorganic interface electron transfer resistance is only 16% compared to the control. The in‐situ biosynthetic Fe‐containing nanoparticles (e.g., FeS) enhance the transmembrane EET and the EET within biofilm, and the peak conductivity increases 3.4‐fold compared to the control. The in‐situ biosynthesis method upregulates the genes involved in energy metabolism and electron transfer from the transcriptome analysis. This study enriches the insights of biosynthetic nanoparticles on electron transfer process, holding promise in bioenergy conversion.
Zhuo Li, Linbin Hu, Qian Fu et al.
Sustainable Energy & Fuels · 2020
Bioelectrochemical systems (BES) possess great potential for simultaneous wastewater treatment and energy recovery.
Sitti Chadijah, Firnanelty Firnanelty, Maswati Baharuddin Baharuddin et al.
Indo. J. Chem. Res. · 2023
Bioelectrochemical Systems (BES) technology is a method for generating electric energy using bacteria as catalysts. The electricity is produced by Microbial Fuel Cells (MFCs), which represent the latest development in biological energy research. This study utilized substrates from banana stems and the bacterium Pseudomonas sp. The research aims to assess the potential of banana stems as a substrate in the MFC system and to determine the effects of adding a combination of an electrolyte solution and an appropriate buffering material to achieve maximum current, potential difference, and power density values. The results showed that the maximum current and potential difference achieved were 1.05 mA and 0.62 V, respectively, with a power density value of 446 mW/m². When combining the KMnO4 electrolyte solution with sodium phosphate buffer, a potential difference of 0.76 V and a current of 1.75 mA were obtained, resulting in a power density value of 911 mW/m². By using the K3[Fe(CN)6] electrolyte solution buffer with potassium phosphate buffer, a current of 1.14 mA was produced, and the power density value reached 406 mW/m².
Mohsen Karimi, Tove Widén, Yvonne Nygård et al.
Biotechnology and Bioengineering · 2021
Abstract Mass transfer has been identified as a major bottleneck in gas fermentation and microbial conversion of carbon dioxide to chemicals. We present a pragmatic and validated Computational Fluid Dynamics (CFD) model for mass transfer in bioelectrochemical systems. Experiments were conducted to measure mixing times and mass transfer in a Duran bottle and an H‐cell. An Eulerian–Eulerian framework with a simplified model for the bubble size distribution (BSD) was developed that utilized only one additional equation for the bubble number density while including the breakup and coalescence. Validations of the CFD model for mixing times showed that the predictions were within the confidence intervals of the measurements, verifying the model's capability in simulating the hydrodynamics. Further validations were performed using constant and varying bubble diameters for the mass transfer. The results showed the benefits of a simplified BSD model, as it yielded improvements of seven and four times in accuracy when assessed against the experimental data for the Duran bottle and H‐cell, respectively. Modeling of the H‐cell predicted that a lower stirring rate improves mass transfer compared with higher stirring rates, which is of great importance when designing microbial cultivation processes. The model offers a feasible framework for advanced modeling of gas fermentation and microbial electrosynthesis.
Kaline Araújo Soares, Jhoni Anderson Schembek Silva, Xin Wang et al.
Fermentation · 2025
Microbial Electrochemistry Technology (MET) leverages the unique process of extracellular electron transfer (EET) between electroactive bacteria (EAB) and electrodes to enable various applications, such as electricity generation, bioremediation, and wastewater treatment. This review highlights significant advancements in EET mechanisms, emphasizing both outward and inward electron transfer pathways mediated by diverse electroactive microorganisms. Notably, the role of electron shuttles, genetic modifications, and innovative electrode materials are discussed as strategies to enhance EET efficiency. Recent studies illustrate the importance of redox-active molecules, such as flavins and metal nanoparticles, in facilitating electron transfer, while genetic engineering has proven effective in optimizing microbial physiology to boost EET rates. The review also examines the impact of electrode materials on microbial attachment and performance, showcasing new composites and nanostructures that enhance power output in microbial fuel cells. By synthesizing the recent findings and proposing emerging research directions, this work provides an overview of EET enhancement strategies, aiming to inform future technological innovations in bioelectrochemical systems (BESs).
Sujal Phadke, Kayla Carpenter, Orianna Bretschger
ECS Meeting Abstracts · 2016
Microbes can be promising catalysts for the conversion of chemical energy to electrical energy in important technologies such as bioelectrochemical systems (BESs) that can support biosensing and wastewater treatment applications. The reliability of BESs depends on the ability of microbes to move electrons from various donor substrates to solid phase electron acceptors through a process known as extracellular electron transfer (EET). Although some of the genes responsible for EET have been identified in a few model prokaryotes, we lack system-wide knowledge about how key genetic interactions evolve under BES conditions and how evolution may affect the long-term operation of these systems. Because mutation and evolution together represent an inevitable aspect of any technology that uses engineered or natural strains as biocatalysts, we need to understand how the genome-wide mutational landscape changes over time under various environmental conditions in BESs. We combined the power of experimental evolution and next generation sequencing to identify the rates as well as patterns of single nucleotide polymorphisms in pure cultures of electrogenic bacteria grown under controlled laboratory conditions with or without BES stimuli. Our analysis reveals how genetic makeup of the biofilm vs. planktonic populations of electrogenic microbes diverges away from each other during evolution. Our approach also allows us to reveal previously hidden players in the genetic networks underlying the EET activity during BES operation. Lastly, our results emphasize the need to characterize evolutionary trajectories of potential biocatalysts in pure cultures as well as multi-species communities to advance the development and efficacy of bioelectrochemical systems.
M. Basitere, Y. Mpentshu, C. Dlangamandla et al.
ASETH-18,ACABES-18 & EBHSSS-18 Nov. 19-20 2018 Cape Town (South Africa) · 2018
— This study reports on the microbial screening for a bio-delipidation system of lipid-rich slaughterhouse wastewater, and on the optimal conditions for lipase production and activity. In this study, swaps were collected from the poultry slaughterhouse discharge point for screening, isolation and characterisation of lipolytic microorganisms using molecular techniques. Bacillus cereus strains AB1 ( BF3 ) and CC-1 (B3O) were identified using 16S rRNA techniques. Maximal lipase production for both strains was observed between pH 6 – 8 and 45 – 60 °C. Optimal lipase activity for BF3 and B30 was achieved at pH 8 and 60 °C, and at pH 8.83 and 45°C, respectively. After partial purification, increased activity was observed for BF3 and B30 strains. Solvents, metal ions and detergents (triclosan and trichlorocarbonilide) affected lipase activity. It was concluded that BF3 and B30 strains were suitable candidates for bio-delipidation systems.
M. Pelić, N. Novakov, V. Djordjević et al.
IOP Conference Series: Earth and Environmental Science · 2021
Wastewater from slaughterhouses in many countries is still discharged into rivers, without having been adequately treated. Such wastewater contains plenty of organic matter which is an ideal source of nutrients for fish, but also for the development of microorganisms. Thus, usage of wastewater in aquaculture could become a health risk for humans, fish due to the introduction of microorganisms into the aquatic environment. In the available literature, there is insufficient data on health and meat safety regarding common carp reared in purified wastewater. The aim of this study was to assess the health and meat safety of common carp cultivated in a fishpond supplemented with slaughterhouse wastewater that was subjected to tertiary treatment. The number of parasites was not significant and not a single parasitic disease was found in this study, but the number of parasite species detected was as expected and typical for carp production. No spring viraemia of carp or koi herpesvirus disease was found. The carp cultivated were in good health and completely safe for human consumption in terms of the presence of microbial contaminants. The safe use of wastewater for fish rearing should be encouraged, but proper treatment of wastewater must be applied before its use.
Eliandra Rodio, Luana Cristina Calliari Leite Rossi, Luiz Felipe Gomes Ferreira et al.
Environmental Technology · 2024
ABSTRACT The deammonification process is an efficient alternative to remove nitrogen from wastewater with a low carbon/nitrogen ratio. However, the reactor configuration and operational factors pose challenges for applications in treatment systems to remove nitrogen from municipal and industrial wastewater on a large scale. To address this gap, this study evaluated a new deammonification strategy using a single-stage membrane aerated biofilm reactor (MABR), operated with continuous flow, under different hydraulic retention times (HRT) in the post-treatment of poultry slaughterhouse wastewater with a low nitrogen load, similar to domestic wastewater. The performance of the MABR reactor and the microbial community was evaluated over a long period at HRTs of 12, 24, and 36 hours, corresponding to nitrogen volumetric loads of 181.0 g N.m−3.d−1, 87.4 g N.m−3.d−1 and 67.8 g N.m−3.d−1. The results show that total nitrogen (TN) removal and the microbial community in the MABR reactor were influenced by changes in HRT. The highest efficiency in TN removal was obtained with an HRT of 24 hours, in which the maximum TN removal efficiency was 77.04%. Candidatus Brocadia and Candidatus Jettenia were the two genera of bacteria with Anammox activity present in the reactor, with relative abundances of 7.27% and 0.74%, respectively. This study helps to deepen the understanding of the application of the single-stage MABR reactor in real wastewater treatment with a low nitrogen load. GRAPHICAL ABSTRACT
Talita Werberich, Adriana Garcia do Amaral, Roselene Maria Schneider et al.
Nativa · 2016
COAGULAÇÃO/FLOCULAÇÃO DE EFLUENTE DE ABATEDOURO UTILIZANDO TORTA DE ALGODÃO COMO COAGULANTENo presente trabalho, teve-se como objetivo avaliar a influência de um produto natural (torta de algodão), quando utilizado como agente coagulante, na remoção de turbidez de efluente proveniente de um abatedouro de suínos. A torta de algodão foi utilizada como coagulante em três formas de preparação, em solução aquosa utilizandose água destilada, solução salina (NaCl – 1M) e em fase sólida. As soluções aquosas foram preparadas no momento do ensaio. Para avaliação do efeito do coagulante no efluente, foram realizados ensaios de coagulação/floculação/sedimentação utilizando o aparelho Jar- test, sendo avaliados os efeitos das dosagens de50, 100, 200, 500, 1000, 1500, 2500 e 5000 mg L-1 de torta de algodão em solução (aquosa e salina) e 50, 100, 200, 500, 1000 e 1500 mg L-1 de torta de algodão em fase sólida. De acordo com os resultados obtidos, observou-se remoções de turbidez quando da utilização da solução salina (41,1%) com dosagem ótima de 1000 mg L-1 e principalmente quando se utiliza a torta de algodão em fase sólida (43,5%) com dosagem ótima de 100 mg L-1, já que há uma maior superfície de contato, podendo este ser considerado um coagulante potencial no tratamento de efluentes agroindustriais.Palavras-chave: coagulante natural, tratamento de efluentes, turbidez. ABSTRACTThe objective of this study was to evaluate the influence of a natural product (cottonseed) when used as a coagulant agent in the turbidity removal from a swine slaughterhouse wastewater. The cottonseed was used as a coagulant in three preparation ways, in aqueous solution using distilled water, saline solution (NaCl - 1M) and in a solid phase. The aqueous solutions were prepared at the time of the test. Coagulation/flocculation/sedimentation tests were performed to evaluate the wastewater coagulant effect using Jar-test device, evaluating the dosages effects of 50, 100, 200, 500, 1000, 1500, 2500 and 5000 mg L-1 cottonseed solution (aqueous and saline) and 50, 100, 200, 500, 1000 and 1500 mg L-1 of solid phase cottonseed. According to the results obtained, the turbidity removals were observed when using saline solution (41.1%) with a great dosage of 1000 mg L-1 and mainly when using solid phase cottonseed (43.5%) with a great dosage of 100 mg L-1, since there is a larger contact surface, which may be considered a potential coagulant in the treatment of agro-industrial wastewater.Keywords: natural coagulant, wastewater treatment, turbidity. DOI: http://dx.doi.org/10.14583/2318-7670.v04n03a03
Irma Lubis, Tri Edhi Budhi Soesilo, Roekmijati W. Soemantojo
Jurnal Manusia dan Lingkungan · 2020
ABSTRAKAir limbah Rumah Potong Hewan (RPH) yang tidak dikelola dengan baik dapat menimbulkan masalah lingkungan dan gangguan pada masyarakat yang bertempat tinggal di sekitar RPH. Sejak RPH X beroperasi pada tahun 2009, pengelolaan air limbah RPH X masih belum berjalan optimal. Hal ini dikarenakan air limbah RPH X masih dilihat sebagai materi yang tidak berguna dan dibuang. Saat ini, praktik pengelolaan air limbah RPH X dilakukan dengan menggabungkan semua air limbah kemudian air limbah tersebut diolah dengan Instalasi Pengolahan Air Limbah (IPAL). Riset ini mencoba menganalisis praktik pemotongan sapi, fasilitas RPH, pengelolaan air limbah RPH, kualitas air limbah RPH, dan dampak limbah RPH pada masyarakat. Riset ini menggunakan pendekatan metode kuantitatif dan kualitatif, melalui observasi, wawancara, kuesioner, dan uji laboratorium. Hasil riset menunjukkan bahwa praktik pemotongan sapi di RPH X termasuk kategori baik, fasilitas RPH X termasuk kategori kurang sesuai dengan persyaratan, pengelolaan air limbah RPH X belum berjalan optimal, kualitas air outlet IPAL telah memenuhi baku mutu, dan dampak limbah RPH X pada masyarakat berupa gangguan bau yang dirasakan oleh 100% responden dan gangguan kesehatan berupa mual yang dirasakan oleh 41% responden. Alternatif peningkatan dalam pengelolaan air limbah RPH yang dapat dilakukan adalah minimisasi air limbah melalui segregasi dan pemanfaatan air limbah RPH.Kata kunci: Rumah Potong Hewan, praktik pemotongan sapi, fasilitas RPH, pengelolaan air limbah RPH, kualitas air limbah RPH, dampak limbah RPH, minimisasiABSTRACTWastewater of slaughterhouse is not managed optimally can cause environmental problems and disruption to communities living around the slaughterhouse. Since slaughterhouse X operates in 2009, wastewater management of the slaughterhouse X is not managed optimally. This is because the wastewater of the slaughterhouse X is seen as useless and discarded material. Currently, the wastewater management of the slaughterhouse X is carried out by mixing all of the wastewater and then the wastewater is treated by Wastewater Treatment Plant (WWTP). This study analyzes practice of cattle slaughtering, slaughterhouse facilities, wastewater management, wastewater quality, impact of slaughterhouse waste. This study exercises quantitative and qualitative methods, through observations, interviews, questionnaires, and laboratory test. The results showed that the practice of cattle slaughtering was categorized into good, the slaughterhouse X facilities were categorized into less suitable, the wastewater management of slaughterhouse X is still not managed optimally, the wastewater quality of WWTP outlet is comply with water quality standards, and the impact of slaughterhouse waste to the communities living around the slaughterhouse is odor disruption felt by 100% of respondents and health issue are nauseous felt by 41% of respondents. An alternative to improving wastewater management of slaughterhouse is minimization through segregation and utilization of slaughterhouse wastewater.
Timoth Mkilima, Gulnur Saspugayeva, Zhazgul Tussupova et al.
Water Environment Research · 2024
Abstract The treatment of slaughterhouse wastewater is a complex task demanding careful consideration due to its challenging nature. Therefore, exploring more sustainable treatment methods for this particular type of wastewater is of utmost significance. This research focused on the impact of electrode materials, specifically graphite and titanium, on the efficiency of microbial fuel cells (MFCs) and electro‐Fenton systems in treating slaughterhouse wastewater. Both graphite and titanium electrodes displayed increasing current density trends, with titanium outperforming graphite. Titanium showed superior electron transfer and current generation (2.2 to 21.2 mA/m 2 ), while graphite ranged from 2.4 to 18.9 mA/m 2 . Titanium consistently exhibited higher power density, indicating better efficiency in converting current to power (0.059 to 22.68 mW/m 2 ), compared to graphite (0.059 to 12.25 mW/m 2 ) over the 48‐h period. In removal efficiency within the MFC system alone, titanium exhibited superior performance over graphite in key parameters, including zinc (45.5% vs. 37.19%), total hardness (39.32% vs. 29.4%), and nitrates (66.87% vs. 55.8%). For the electro‐Fenton system with a graphite electrode, the removal efficiency ranged from 34.1% to 87.5%, with an average efficiency of approximately 56.2%. This variability underscores fluctuations in the efficacy of the graphite electrode across diverse wastewater treatment scenarios. On the other hand, the electro‐Fenton system employing a titanium electrode showed removal efficiency values ranging from 26.53% to 89.99%, with an average efficiency of about 68.4%. The titanium electrode exhibits both a comparatively higher and more consistent removal efficiency across the evaluated scenarios. On the other hand, the integrated system achieved more than 90% removal efficiency from most of the parameters. The study underscores the intricate nature of slaughterhouse wastewater treatment, emphasizing the need for sustainable approaches. Practitioner Points Microbial fuel cell (MFC) and electro‐Fenton were investigated for slaughterhouse wastewater treatment. The MFC microbial activity started to decrease after 24 h. The integrated system achieved up to 99.8% removal efficiency (RE) for total coliform bacteria. Up to 99.4% of RE was also achieved for total suspended solids (TSS). The integrated system highly improved RE of the pollutants.
Semba Michael, Chrisogoni Paschal, Thomas Kivevele et al.
Water Practice and Technology · 2020
Abstract The present study engaged onsite operations and laboratory analysis for Mwanza City Slaughterhouse (MCS) wastewater to improve the efficiency of wastewater treatment of a newly installed facility. The MCS wastewater treatment facility is integrated with various units-biodigester, aeration unit, retention, clarifier, and a constructed wetland. During the initial runs, the MCS facility removed 87.5%, 92.2%, 43%, and 65.4% of effluent biochemical oxygen demand (BOD5), chemical oxygen demand (COD), ammonium, and nitrate, respectively. After conducting effective plant operations for five months, the removal efficiencies of BOD5, COD, ammonium, and nitrate improved to 97.4%, 98.3%, 97.4%, and 97.6%, respectively. In the present study, the unit-by-unit performance values achieved as a result of alterations to the facility's running conditions are presented. The MCS wastewater treatment facility was found to be energy-positive, as it produced an average of 158.2 m3 biogas per day. This amount of biogas, if converted to electricity, would be sufficient to run the facility operations. Generally, the MCS wastewater treatment facility attained the best performance as per design, achieving the effluent levels recommended by the Tanzania Bureau of Standards (TBS).
Que Nguyen Ho, Kyosuke Mitsuoka, Naoko Yoshida
Environmental Research · 2024
Chao-Chin Chang, Shiue-Lin Li, Anyi Hu et al.
Chemosphere · 2021
Oscar Guerrero-Sodric, Juan Antonio Baeza, Albert Guisasola
Water Research · 2024
Dan Chen, Lizhuang Yang, Zhiling Li et al.
Environmental Research · 2021
Shabnam Ahmadi, Abbas Rezaee, Soumya Ghosh et al.
Journal of Environmental Chemical Engineering · 2023
Matthew D. Yates, Lina J. Bird, Brian J. Eddie et al.
Bioelectrochemistry · 2021
Bacterial extracellular electron transfer (EET) is envisioned for use in applied biotechnologies, necessitating electrochemical characterization of natural and engineered electroactive biofilms under conditions similar to the target application, including small-scale biosensing or biosynthesis platforms, which is often distinct from standard 100 mL-scale stirred-batch bioelectrochemical test platforms used in the laboratory. Here, we adapted an eight chamber, nanoliter volume (500 nL) electrochemical flow cell to grow biofilms of both natural (Biocathode MCL community, Marinobacter atlanticus, and Shewanella oneidensis MR1) or genetically modified (S. oneidensis ΔMtr and S. oneidensis ΔMtr + pLB2) electroactive bacteria on electrodes held at a constant potential. Maximum current density achieved by unmodified strains was similar between the nano- and milliliter-scale reactors. However, S. oneidensis biofilms engineered to activate EET upon exposure to 2,4-diacetylphloroglucinol (DAPG) produced current at wild-type levels in the stirred-batch reactor, but not in the nanoliter flow cell. We hypothesize this was due to differences in mass transport of DAPG, naturally-produced soluble redox mediators, and oxygen between the two reactor types. Results presented here demonstrate, for the first time, nanoliter scale chronoamperometry and cyclic voltammetry of a range of electroactive bacteria in a three-electrode reactor system towards development of miniaturized, and potentially high throughput, bioelectrochemical platforms.
JunLin Wen, DaiGui He, SongQing Luo et al.
Science China Technological Sciences · 2023
Duyen M. Pham, Sujan Dey, Arata Katayama
International Journal of Biological Macromolecules · 2022
Extracellular electron transfer material (EETM) has increasingly attracted attentions for the enhancing effect on multiple microbial reactions. Especially, EETM is known to be essential to activate the energy network in non-electroactive bacteria. It is motivated to find out an EETM which is natural-based, environmentally friendly, and easily produced at large-scale. In this study, Bombyx mori silk is found, for the first time, to function as an EETM by using an EETM-dependent pentachlorophenol (PCP) dechlorinating anaerobic microbial culture. Subsequently, by dividing fibroin fiber into different soluble/insoluble fractions and correlating their EET functions with their structural properties based on various spectroscopic analyses, the β-sheet configuration is suggested as an essential structure supporting the EET function of silk materials. The analyses also suggested the involvement of sulfur-containing amino acids in this function. The EET function is not degraded by boiling or acid/alkaline treatments and the material can be utilized multiple times, although it is susceptible to UV irradiation. Bombyx mori silk also enhance other microbial reactions, including Fe(III)OOH reduction, CO 2 reduction to acetate, and nitrogen fixation. This discovery provides a basis for developing biotechnology for environmental remediation, global warming reduction, and biofertilizer production using Bombyx mori silk and its wastes.
Wei Guo, Yingying Chen, Jiayi Wang et al.
Bioelectrochemistry · 2024
The structure and surface physicochemical properties of anode play a crucial role in microbial fuel cells (MFCs). To enhance the enrichment of exoelectrogen and facilitate extracellular electron transfer (EET), a three-dimensional macroporous graphene aerogel with polydopamine coating was successfully introduced to modify carbon brush (PGA/CB). The three-dimensional graphene aerogel (GA) with micrometer pores improved the space utilization efficiency of microorganisms. Polydopamine (PDA) coating enhanced the physicochemical properties of the electrode surface by introducing abundant functional groups and nitrogen-containing active sites. MFCs equipped with PGA/CB anodes (PGA/CB-MFCs) demonstrated superior power generation compared to GA/CB-MFCs and CB-MFCs (MFCs with GA/CB and CB anodes respectively), including a 23.0 % and 30.1 % reduction in start-up time, and an increase in maximum power density by 2.43 and 1.24 times respectively. The higher bioelectrochemical activity exhibited by the biofilm of PGA/CB anode and the promoted riboflavin secretion by PGA modification imply the enhanced EET efficiency. 16S rRNA high-throughput sequence analysis of the biofilms revealed successful enrichment of Geobacter on PGA/CB anodes. These findings not only validate the positive impact of the synergistic effects between GA and PDA in promoting EET and improving MFC performance but also provide valuable insights for electrode design in other bioelectrochemical systems.
Chelsea Catania, Amruta A. Karbelkar, Ariel L. Furst
Joule · 2021
Jin Zhu, Baoguo Wang, Yixin Zhang et al.
Biosensors and Bioelectronics · 2023
Bioelectrical interfaces made of living electroactive bacteria (EAB) provide a unique opportunity to bridge biotic and abiotic systems, enabling the reprogramming of electrochemical biosensing. To develop these biosensors, principles from synthetic biology and electrode materials are being combined to engineer EAB as dynamic and responsive transducers with emerging, programmable functionalities. This review discusses the bioengineering of EAB to design active sensing parts and electrically connective interfaces on electrodes, which can be applied to construct smart electrochemical biosensors. In detail, by revisiting the electron transfer mechanism of electroactive microorganisms, engineering strategies of EAB cells for biotargets recognition, sensing circuit construction, and electrical signal routing, engineered EAB have demonstrated impressive capabilities in designing active sensing elements and developing electrically conductive interfaces on electrodes. Thus, integration of engineered EAB into electrochemical biosensors presents a promising avenue for advancing bioelectronics research. These hybridized systems equipped with engineered EAB can promote the field of electrochemical biosensing, with applications in environmental monitoring, health monitoring, green manufacturing, and other analytical fields. Finally, this review considers the prospects and challenges of the development of EAB-based electrochemical biosensors, identifying potential future applications.
Niloufar Fattahi, Jeffrey Reed, Evan Heronemus et al.
Bioelectrochemistry · 2024
Loss of bioelectrochemical activity in low resource environments or from chemical toxin exposure is a significant limitation in microbial electrochemical cells (MxCs), necessitating the development of materials that can stabilize and protect electroactive biofilms. Here, polyethylene glycol (PEG) hydrogels were designed as protective coatings over anodic biofilms, and the effect of the hydrogel coatings on biofilm viability under oligotrophic conditions and ammonia-N (NH 4 + -N) shocks was investigated. Hydrogel deposition occurred through polymerization of PEG divinyl sulfone and PEG tetrathiol precursor molecules, generating crosslinked PEG coatings with long-term hydrolytic stability between pH values of 3 and 10. Simultaneous monitoring of coated and uncoated electrodes co-located within the same MxC anode chamber confirmed that the hydrogel did not compromise biofilm viability, while the coated anode sustained nearly a 4 × higher current density (0.44 A/m 2 ) compared to the uncoated anode (0.12 A/m 2 ) under oligotrophic conditions. Chemical interactions between NH 4 + -N and PEG hydrogels revealed that the hydrogels provided a diffusive barrier to NH 4 + -N transport. This enabled PEG-coated biofilms to generate higher current densities during NH 4 + -N shocks and faster recovery afterwards. These results indicate that PEG-based coatings can expand the non-ideal chemical environments that electroactive biofilms can reliably operate in.
Yi Li, Weizhong Li, Yazhi Yang et al.
Biosensors and Bioelectronics · 2022
Mohamad Afiq Mohd Asrul, Mohd Farid Atan, Hafizah Abdul Halim Yun et al.
International Journal of Hydrogen Energy · 2025
Thi Pham Phan, Quang Huy Hoang Phan, Phan Khanh Thinh Nguyen
Fuel · 2024
Hong-zhou Liu, Tie-zhu Chen, Jian-chang Li
Journal of Power Sources · 2025
Xiaochi Zheng, Long Chen, Shaohui Zhang et al.
Journal of Water Process Engineering · 2024
Peike Wang, Min Zhang, Tianyu Yan et al.
Renewable Energy · 2025
Matthew J. Berens, Tobin W. Deen, Chan Lan Chun
Chemosphere · 2024
Zhang Cheng, Shiyun Yao, Heyang Yuan
Water Research · 2021
Mechanistic and data-driven models have been developed to provide predictive insights into the design and optimization of engineered bioprocesses. These two modeling strategies can be combined to form hybrid models to address the issues of parameter identifiability and prediction interpretability. Herein, we developed a novel and robust hybrid modeling strategy by incorporating microbial population dynamics into model construction. The hybrid model was constructed using bioelectrochemical systems (BES) as a platform system. We collected 77 samples from 13 publications, in which the BES were operated under diverse conditions, and performed holistic processing of the 16S rRNA amplicon sequencing data. Community analysis revealed core populations composed of putative electroactive taxa Geobacter, Desulfovibrio, Pseudomonas, and Acinetobacter. Primary Bayesian networks were trained with the core populations and environmental parameters, and directed Bayesian networks were trained by defining the operating parameters to improve the prediction interpretability. Both networks were validated with Bray-Curtis similarly, relative root-mean-square error (RMSE), and a null model. A hybrid model was developed by first building a three-population mechanistic component and subsequently feeding the estimated microbial kinetic parameters into network training. The hybrid model generated a simulated community that shared a Bray-Curtis similarity of 72% with the actual microbial community at the genus level and an average relative RMSE of 7% for individual taxa. When examined with additional samples that were not included in network training, the hybrid model achieved accurate prediction of current production with a relative error-based RMSE of 0.8 and outperformed the data-driven models. The genomics-enabled hybrid modeling strategy represents a significant step toward robust simulation of a variety of engineered bioprocesses.