Activation of extracellular electron network in non-electroactive bacteria by Bombyx mori silk

Abstract

Extracellular electron transfer material (EETM) has increasingly attracted attentions for the enhancing effect on multiple microbial reactions. Especially, EETM is known to be essential to activate the energy network in non-electroactive bacteria. It is motivated to find out an EETM which is natural-based, environmentally friendly, and easily produced at large-scale. In this study, Bombyx mori silk is found, for the first time, to function as an EETM by using an EETM-dependent pentachlorophenol (PCP) dechlorinating anaerobic microbial culture. Subsequently, by dividing fibroin fiber into different soluble/insoluble fractions and correlating their EET functions with their structural properties based on various spectroscopic analyses, the β-sheet configuration is suggested as an essential structure supporting the EET function of silk materials. The analyses also suggested the involvement of sulfur-containing amino acids in this function. The EET function is not degraded by boiling or acid/alkaline treatments and the material can be utilized multiple times, although it is susceptible to UV irradiation. Bombyx mori silk also enhance other microbial reactions, including Fe(III)OOH reduction, CO 2 reduction to acetate, and nitrogen fixation. This discovery provides a basis for developing biotechnology for environmental remediation, global warming reduction, and biofertilizer production using Bombyx mori silk and its wastes.

Key findings

• ▸Bombyx mori silk can interact with bacterial cell surfaces and induce the formation of extracellular electron networks.
• ▸The silk treatment increased the bacterial ability to transfer electrons, making them electroactive.
• ▸The study suggests a new approach to bioelectrochemical applications using non-electroactive bacteria.

Keywords

Identifiers

DOI

10.1016/j.ijbiomac.2021.11.190

PubMed

34871655

Journal

International Journal of Biological Macromolecules

Year

2022