MESSAI.iO

Electrogen · Denitrifier · Contaminant pathogen

Pseudomonas aeruginosa PA14

Pseudomonadota · Gammaproteobacteria · Pseudomonadaceae

ElectroactiveEET outwardBSL-2Partial record
  • Phenazine producer
  • Pathogen
  • Shuttle mediated
  • Tier 2
Outward
Electron transfer
electroactive
37°C
Optimal temperature
grows 20–42 °C
7
Optimal pH
grows pH 6–8

01 · Identity

Identity

Where this organism sits in the tree of life, how to obtain it, and what a cell looks like.

Lineage

  1. domainBacteria
  2. ›phylumPseudomonadota
  3. ›classGammaproteobacteria
  4. ›orderPseudomonadales
  5. ›familyPseudomonadaceae
  6. ›genusPseudomonas
  7. ›speciesPseudomonas aeruginosa PA14

Culture collections & accessions

NCBI taxon
208963
Genome
GCF_000014625.1

Cell morphology

Rod, Gram-negative (diderm)

  • Motile: yes
  • Spore-forming: no

02 · Electron transfer

Electron transfer

How electrons cross the cell envelope — the property that makes a microbe useful in an electrochemical system.

ElectroactiveEET outward
CELLELECTRODESOLUBLE SHUTTLESpyocyaninphenazine-1…phenazine-1…e⁻
Electrons flow outward: cell → electrode (exoelectrogen)

Mechanisms

  • Outer-membrane cytochromes: no
  • Conductive pili / nanowires: no
  • Endogenous shuttles: yes

03 · Growth envelope

Growth envelope

The conditions this organism tolerates and what it eats and breathes — the operating window for a reactor.

MesophileFacultative anaerobeChemoorganoheterotroph
Temperature37 (20–42) °C
0100 °C
pH7 (6–8)
014

Energy metabolism

Electron donors

  • Glucose
  • Acetate

Electron acceptors

  • O2
  • Nitrate
  • Anode

Carbon sources

  • Glucose
  • Acetate
  • Succinate
  • Glycerol
  • amino acids
  • Respiratory: yes

04 · Performance

Performance

Reported electrochemical output, the role it plays in a reactor, and what it produces.

Moderate electrogen
200mW/m2
Max power density
range 50–800 · per projected anode area

Role in a reactor

Anode: supporting anode biofilm

05 · Ecology & biofilm

Ecology & biofilm

How it lives on an electrode, who it partners with, and where it is found in nature.

Biofilm

  • Forms biofilm: yes

Preferred electrode materials

  • carbon cloth
  • Graphite
Quorum sensing molecules
  • 3-oxo-C12-HSL
  • C4-HSL
  • PQS

Ecosystems

  • Host associated
  • Soil
  • Freshwater

Isolation sources

  • clinical burn wound
  • Soil
  • Water

06 · Applications, engineering & safety

Applications, engineering & safety

Where it has been put to work, how tractable it is to engineer, and what handling it requires.

Applications (2)

  • Bioelectricity generation
    TRL 3/9
  • Biosensor
    TRL 3/9

Engineering

Engineered strains publishedIndustrial relevance 2/5

Reactor compatibility

  • MFC single chamber
  • MFC dual chamber

Genetics

  • Genetically tractable: yes

Key EET genes

  • phzA-G
  • phzM
  • phzS

qPCR targets

  • phzM
  • phzS

Biosafety

BSL-2
  • Pathogen: yes
  • Carries concerning ARGs: yes

Opportunistic human pathogen; major nosocomial concern. Use BSL-2 containment.

Modeling assets

Linked GEM: iJN1463

07 · Sources & data quality

Sources & data quality

Every value above traces to a citation. This is how complete and how confident the record is.

Record quality

PartialMedium confidenceCurated: manual

Literature reviewed 2026-04-26 · schema v1.2.0 · updated 2026-04-28 · imported 2026-04-28

References (2)

  1. Rabaey K, et al. (2005). Microbial phenazine production enhances electron transfer. Environ Sci Technol 39:3401-3408.doi:10.1021/es048563o
  2. Rabaey K, et al. (2005). Microbial phenazine production enhances electron transfer. Environ Sci Technol 39:3401-3408.doi:10.1021/es048563o