Nanodiamond-embedded tungsten disulfide nanosheets for electrochemical detection of a bacterial metabolite pyocyanin

Abstract

Abstract Pyocyanin (PYO) is a distinctive electroactive virulence factor secreted by Pseudomonas aeruginosa , a bacterium implicated in a spectrum of severe human infections, particularly in susceptible and critically ill patients. The rapid and accurate diagnosis of infectious agents is crucial for prescribing effective antibiotics and ensuring successful treatment outcomes in patients facing critical conditions. Due to its redox-active nature, PYO actively participates in electron acceptance and donation processes at the electrode surface, making it well-suited for electrochemical detection. To enhance this detection capability, we utilized a physically cross-linked methodology to embellish the surface of a working electrode with a nanocomposite composed of tungsten disulfide nanosheets supported by nanodiamond (WS 2 NSs-ND). This innovative approach was implemented to enhance electron transport, and sample absorption on the electrode surface with a sensitivity of 0.38 µA µM − 1 cmdue to its increased number of active sites. Furthermore, WS 2 NSs-ND demonstrated remarkable electrochemical performance when applied to real sample human urine, yielding RSD of ± 2%. This underscores the reliability and robustness of WS 2 NSs-ND/GCE for the accurate detection of PYO in diverse biological samples, showcasing its potential for clinical applications.

Keywords

Identifiers

DOI

10.21203/rs.3.rs-5406178/v1

Journal

Research Square

Year

2024