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This study demonstrates an efficient repeated medium replacement method to enrich a mixed bio-cathode denitrifying culture (MBD) in microbial fuel cells for nitrogen removal without added organic carbon. The MBD achieved 69.99% NO₃⁻-N removal efficiency and stable voltage output of 120 mV over 380 hours. A facultative anaerobic strain Lyy (Stutzerimonas stutzeri) isolated from MBD exhibited >98% denitrification efficiency across broad C/N ratios (2–12) and KNO₃ concentrations (500–3000 mg/L), with electron shuttles facilitating electrotrophic denitrification.

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What they did

System
MFC

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Abstract

Recent advancements in microbial fuel cells (MFC) technology have significantly contributed to the development of bio-cathode denitrification as a promising method for eco-friendly wastewater treatment. This study utilized an efficient repeated replacement method to enrich a mixed bio-cathode denitrifying culture (MBD) within a bio-cathode MFC, achieving a stable maximum output voltage of 120 ± 5 mV and a NO 3 - -N removal efficiency of 69.99 ± 0.60%. The electrotrophic denitrification process appears to be facilitated by electron shuttles. Microbial community analysis revealed a predominance of Proteobacteria , with Paracoccus and Pseudomonas as functional genera. Additionally, the isolated strain Lyy (belonging to Stutzerimonas ) from MBD demonstrated exceptional denitrification efficiencies exceeding 98% when treating wastewater with a broad range of C/N (2-12) ratios and KNO 3 concentrations (500-3000 mg/L) within 60 h. These results demonstrated the effectiveness of the repeated replacement method in enriching bio-cathode denitrifiers and advancing MFC application in sustainable wastewater management.

Keywords

Microbial fuel cellCathodeBiochemical engineeringEnvironmental chemistryNanotechnology

Identifiers

DOI
10.1016/j.isci.2024.110965
PubMed
39435140
Journal
iScience
Year
2024