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This MEC study investigates Anaerobic ammonium oxidation, Carbon metabolism, Microbial community. Key performance metrics include voltage output.

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What they did

System
MEC

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Abstract

Anaerobic ammonium-oxidizing bacteria (AnAOB) present significant enrichment challenges due to slow growth kinetics and extended cultivation periods. This study employed single-chamber microbial electrolysis cells (MECs) to investigate substrate conversion rates, microbial community dynamics, and bacterial morphological adaptations in mixed consortia (incorporating nitrifiers, denitrifiers, and AnAOB) under varying applied voltages (0-1.2 V). Key results demonstrated that at 0.6 V, ammonium removal efficiency nearly doubled compared to the 0 V baseline, with AnAOB relative abundance increasing from 5.78 ± 0.38% to 12.34 ± 0.60% while denitrifiers decreased from 9.69 ± 1.33% to 2.84 ± 0.14%. Voltage escalation to 1.2 V induced proliferation of denitrifiers, which coexisted with AnAOB under carbon-limited conditions. During actual wastewater operation, ammonium degradation rates declined to 73% (Cycle 1) and 68% (Cycle 2), yet AnAOB maintained functional dominance at 12.56 ± 0.40% relative abundance. These findings provide valuable insights for AnAOB enrichment from complex communities, demonstrating voltage optimization's potential to enhance syntrophic interactions and intensify nitrogen removal pathways.

Keywords

Anaerobic ammonium oxidationCarbon metabolismMicrobial communityMicrobial electrolysisNitrogen removal

Identifiers

PubMed
41231292
Journal
Environmental monitoring and assessment
Year
2025