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A Gram-positive isolate, Thermincola potens JR, was studied in a microbial fuel cell (MFC) and found to produce no evidence of soluble redox-active components being secreted into the surrounding medium, despite having a functional electron transport mechanism.

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What they did

System
MFC

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Abstract

of important components deficient mutant [39] showed that the mutant had a much-reduced ca­ in the electron transport mechanism from cells to the anode in Geobacter pacity at producing current, but not zero, probably due to the secretion MFCs has been provided by Lovley [37]. Metabolism of electron rich of flavin molecules, suggesting that some species may use all three (reduced) substrate such as acetate or lactate drives the production of transport mechanisms shown in Fig. 4. The majority of species studied to reduced NADH from NAD+ within the cell. In order for the cell to date are Gram negative organisms. However, Thermincola potens strain maintain reducing power, it must re-oxidise NAD+ by abstracting elec­ JR, is a Gram-positive isolate obtained from the anode surface of a mi­ trons by using dehydrogenase and the cytochrome system consisting of crobial fuel cell [40]. Despite careful study this species produced no quinone/menaquinone pool, periplasmic proteins MacA, PpcA, and evidence of any soluble redox-active components being secreted into the outer membrane proteins, OmcE and OmcS. Together these are able to surrounding medium. Confocal microscopy revealed highly stratified transport the electrons by a series of redox reactions spanning the inner biofilms in which the cells contacting the electrode surface were cytoplasmic membrane across the periplasmic space until the electron is disproportionately viable relative to the rest of the biofilm. Furthermore, conducted across the outer membrane to the anode electrode via the there was no correlation between biofilm thickness and power produc­ outer membrane cytochromes OmcE and OmcS (Fig. 4b). Further tion, suggesting that cells in contact with the electrode were primarily

Key findings

  • The mutant had a much-reduced capacity at producing current in the electron transport mechanism from cells to the anode in Geobacter MFCs.
  • The majority of species studied to date are Gram-negative organisms, but Thermincola potens JR is a Gram-positive isolate.
  • Confocal microscopy revealed highly stratified biofilms in the MFC.

Keywords

MFC3D modelingextracted

Identifiers

Journal
homepage: www.sciencedirect.com/journal/biofilm
Year
2019