MESSAI.iO
Generic MFCRepresentative model
Click to animate flow
loading 3D model…

Representative MFC — matched on the paper’s system type only, not its reactor or geometry.

Extraction

Reported parameters

No values extracted from this paper yet.

Open in lab for full controls, parameter editing, and template overlays.

Open in lab →

What they did

System
MFC

What worked

No outcome metrics extracted yet.

Abstract

Flavins regulate the rate and direction of extracellular electron transfer (EET) in Shewanella oneidensis. However, low concentration of endogenously secreted flavins by the wild-type S. oneidensis MR-1 limits its EET efficiency in bioelectrochemical systems (BES). Herein, a synthetic flavin biosynthesis pathway from Bacillus subtilis was heterologously expressed in S. oneidensis MR-1, resulting in ∼25.7 times' increase in secreted flavin concentration. This synthetic flavin module enabled enhanced bidirectional EET rate of MR-1, in which its maximum power output in microbial fuel cells increased ∼13.2 times (from 16.4 to 233.0 mW/m²), and the inward current increased ∼15.5 times (from 15.5 to 255.3 μA/cm²).

Keywords

Shewanella oneidensisFlavin groupShewanellaBacillus subtilisElectron transferChemistryBiochemistryExtracellularBacteriaBiophysicsBiologyEnzymeOrganic chemistryGenetics

Identifiers

DOI
10.1021/sb500331x
Journal
ACS Synthetic Biology
Year
2015