Development of a Bacillus subtilis expression system using the improved Pglv promoter

Abstract

B. subtilis is an important organism in the biotechnological application. The efficient expression system is desirable in production of recombinant gene products in B. subtilis. Recently, we developed a new inducible expression system in B. subtilis, which directed by B. subtilis maltose utilization operon promoter Pglv. The system demonstrated high-level expression for target proteins in B. subtilis when induced by maltose. However, the system was markedly repressed by glucose. This limited the application of the system as a high-expression tool in biotechnology field. The aim of this study was to further improve the Pglv promoter system and enhance its expression strength.

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Identifiers

DOI

10.1186/1475-2859-9-55

PubMed

20618987

Journal

Microbial Cell Factories

Year

2009