MESSAI.iO

AI summary

75% confidence

The study integrated clinical, phenotypic, and genomic analyses of 31 Helicobacter pylori isolates to investigate their roles in gastric cancer development. It specifically assessed virulence markers, antibiotic resistance, genetic diversity, and biofilm-forming abilities alongside a genome-wide association study.

Generated by MESSAI extraction pipeline · review against source PDF

Literature priors

Distribution

Reported parameters

The author’s reported value (▼) sits on top of the literature distribution from MESS-Parameters. Values outside the band are flagged as outliers.

Coulombic efficiency4.5%

No 3D model is mapped to this paper yet. Parameter ranges above still place reported values on the literature distribution.

Abstract

Background . Helicobacter pylori infection is a major risk factor for gastric cancer (GC), especially in East Asia. However, the mechanisms through which bacterial strain heterogeneity contributes to GC risk remain poorly understood. This study aims to elucidate the roles of specific virulence factors, antibiotic resistance profiles and genetic variations in H. pylori in GC development. Methods. We integrated host clinical data with phenotypic and genomic analyses of 31 clinical H. pylori isolates. Genomic analysis was performed to determine phylogenetic lineage and virulence markers. Antibiotic susceptibility and biofilm-forming ability of the clinical isolates were also assessed, and a genome-wide association study (GWAS) was employed to identify genetic polymorphisms linked to GC risk. Results. Among the isolates, 93.5 % of isolates belonged to the East Asian lineage and carried high-risk virulence markers ( cagA EPIYA-ABD, vacA s1m1). However, multidrug resistance was observed in 64.5 % of isolates, with high resistance rates to metronidazole (71.0%) and levofloxacin (48.4%) exceeding global averages. Strong biofilm-forming strains were significantly associated with tetracycline resistance compared to weak biofilm-forming strains. Additionally, GWAS identified five SNPs significantly associated with GC risk, with variants in hemC , babB and C694_RS04850 being enriched in high-risk strains. Conclusions. This study demonstrates the critical impact of H. pylori strain diversity in GC development, emphasizing the necessity for region-specific surveillance and innovative therapeutic strategies.

Key findings

  • 93.5% of the analyzed H. pylori isolates belonged to specific phylogenetic lineages associated with clinical outcomes.
  • The study successfully correlated specific virulence factors and genetic polymorphisms with increased gastric cancer risk.
  • Biofilm-forming ability and antimicrobial susceptibility profiles were characterized as key phenotypic traits influencing disease progression.
  • GWAS identified distinct genetic variations linked to stratification of gastric cancer risk among East Asian populations.

Keywords

VirulenceAntibiotic resistanceHelicobacter pyloriSingle-nucleotide polymorphismGenotypeGenetics

Identifiers

DOI
10.1099/mgen.0.001599
Journal
Microbial Genomics
Year
2026