Archaea catalyze iron-dependent anaerobic oxidation of methane

Abstract

Anaerobic oxidation of methane (AOM) is crucial for controlling the emission of this potent greenhouse gas to the atmosphere. Nitrite-, nitrate-, and sulfate-dependent methane oxidation is well-documented, but AOM coupled to the reduction of oxidized metals has so far been demonstrated only in environmental samples. Here, using a freshwater enrichment culture, we show that archaea of the order Methanosarcinales , related to " Candidatus Methanoperedens nitroreducens," couple the reduction of environmentally relevant forms of Fe 3+ and Mn 4+ to the oxidation of methane. We obtained an enrichment culture of these archaea under anaerobic, nitrate-reducing conditions with a continuous supply of methane. Via batch incubations using [ 13 C]methane, we demonstrated that soluble ferric iron (Fe 3+ , as Fe-citrate) and nanoparticulate forms of Fe 3+ and Mn 4+ supported methane-oxidizing activity. CO 2 and ferrous iron (Fe 2+ ) were produced in stoichiometric amounts. Our study connects the previous finding of iron-dependent AOM to microorganisms detected in numerous habitats worldwide. Consequently, it enables a better understanding of the interaction between the biogeochemical cycles of iron and methane.

Keywords

Identifiers

DOI

10.1073/pnas.1609534113

PubMed

27791118

Journal

Proceedings of the National Academy of Sciences

Year

2016