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MES research in microbial, electrochemical. In enzyme-based biosensors, Ag+ eluted from the reference electrode inhibits the...

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Abstract

In enzyme-based biosensors, Ag+ eluted from the reference electrode inhibits the enzyme activity. Herein, to suppress the inhibition of bilirubin oxidase (BOD) by Ag+, kinetic analysis was used to examine the effect of Ag+ on the activity of BOD. It was confirmed that the addition of Ag+ decreased the bioelectrocatalytic activity of BOD. Atomic absorption spectroscopy (AAS) suggested that Ag+ was attached to BOD. Moreover, the changes in the visible absorption spectra after Ag+ addition showed that Ag+ was bound to the type I Cu sites in BOD. During oxygen reduction by BOD, the direct-electron-transfer-type bioelectrocatalytic current decreased after Ag+ was added. The decay of the catalytic current was evaluated using kinetic analysis (assuming a pseudo-first-order reaction). Based on the analysis, the inhibition of BOD was suppressed when the Ag+ concentration was below 0.1 µM. Referring to the solubility product of AgCl, Cl− at a concentration of 1 mM suppressed the inhibition of the enzymatic activity by 95%.

Keywords

Bilirubin oxidaseElectron transferCatalysisNuclear chemistryElectrochemistryAbsorption spectroscopy

Identifiers

DOI
10.1007/s44211-022-00111-9
Journal
Analytical Sciences
Year
2022