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MES research in electrode, performance. Abstract This amperometric technique for the determi...

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Abstract

Abstract This amperometric technique for the determination of enzyme activity is based on detecting a decrease in the concentration of the NADH co-factor of the enzyme reaction. A glassy carbon electrode, modified by adsorption of Mg2+ and NADH, is used to measure the anodic peak current that corresponds to the oxidation of NADH. We found no significant difference between the enzyme activity of lactate dehydrogenase (E.C.1.1.1.27) preparations as measured by the above amperometric technique and by a spectrophotometric method.

Keywords

AmperometryGlassy carbonElectrochemistryLactate dehydrogenaseElectrodeDehydrogenase

Identifiers

DOI
10.1093/clinchem/30.11.1780
Journal
Clinical Chemistry
Year
1984