Design of an insulin tracer protein-based biosensor for insulin determination

Abstract

The regulation of blood glucose levels is controlled by insulin, which is produced by the pancreatic beta system. Inadequate synthesis of beta insulin, results in elevated glucose levels, a condition known as diabetes, which can lead to various chronic health issues. In recent times, the diagnosis of diabetes, particularly type 1, has shifted towards the direct measurement of insulin levels. To facilitate this, an immunosensor was created to enable rapid and sensitive examination of insulin levels, with the goal of improving the quality for life for diabetic patients. For this purpose, an insulin tracer protein based biosensor was designed for the determination of insulin at all solutions. For determination of insulin, electrobiochemical analyses were performed. The insulin biosensor cyclic woltammogram was obtained between -0,1 and 0,6 V potantial. At 0,45 V was found as the anodic peak side for determination the insulin. Optimisation and characterisation studies performed at 0,45 V with differential pulse voltammetry. The study successfully identified stable and easy-to-use insulin concentrations, indicating the potential of the newly developed immunosensor for applications in clinical biochemistry laboratories.

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Identifiers

DOI

10.37990/medr.1466027

Journal

Medical Records

Year

2024