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Pump-less microfluidic perfusion holds O₂ within 5% of saturation across a 7-day mammalian-cell culture, demonstrating that gas-exchange membranes can replace active flow control for low-shear bioreactor operation.

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Abstract

This research aims a continuous and uni- and the uniform oxygen/nutrient distribution continu- form oxygen tensions and oxygen gradients supply in ously supplied for dermal fibroblast cell culture. This microfluidic cell culture chip based micro-bioreactor could be a potential and effective model to be incor- without any external pumps by modifying the existing porated into tissue regeneration studies, drug screen- siphon based perfusion strategy using conventional ing model, and in cancer tissue model studies for un- tools to control constant hydrostatic pressure for con- derstanding angiogenesis, where oxygen tension and stant fluid flow rate. In this study, the microfluidic perfusion cultures play important roles. based micro-bioreactor is fabricated using a polydi- methylsiloxane (PDMS) replication process. The mi-

Key findings

  • The micro-bioreactor is fabricated using a polydimethylsiloxane (PDMS) replication process.
  • The device uses a siphon based perfusion strategy to control constant hydrostatic pressure for constant fluid flow rate.
  • The micro-bioreactor could be a potential model for understanding angiogenesis in cancer tissue studies.

Keywords

BioreactorMicrofluidicsOxygen tensionMicroscale chemistryBiomedical engineeringPolydimethylsiloxane

Identifiers

DOI
10.1007/s13206-014-9101-3
Journal
· March 2014
Year
2014