Microbial conversion of p-hydroxyphenylacetic acid to homogentisic acid
E. R. Blakley
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90% confidenceMES research in bacteria. An unidentified bacterium degrades p-hydroxyphenylacetic acid by a pathway which involves h...
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Abstract
An unidentified bacterium degrades p-hydroxyphenylacetic acid by a pathway which involves homogentisic acid as an intermediate. Extracts of cells grown on p-hydroxyphenylacetic acid contain an enzyme that converts p-hydroxyphenylacetic acid to homogentisic acid. The enzyme has been partially purified by ammonium sulfate fractionation and some of its properties examined. The complete oxidation of 1 mole of p-hydroxyphenylacetic acid requires 1 mole of either NADH or NADPH, and 1 mole of oxygen, and results in the production of 1 mole of homogentisic acid. The enzyme preparation has a high specificity for p-hydroxyphenylacetic acid, but has about 25% activity with p-hydroxyphenylpyruvic acid.
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Identifiers
- Journal
- Canadian Journal of Microbiology
- Year
- 1972