Microbial fuel cell biosensor for the determination of biochemical oxygen demand of wastewater samples containing readily and slowly biodegradable organics

Abstract

Abstract Objectives Single-chamber air cathode microbial fuel cells (MFCs) were applied as biosensors for biochemical oxygen demand (BOD) measurement of real wastewaters with considerable suspended and/or slowly biodegradable organic content. Results The measurement method consists of batch sample injection, continuous measurement of cell voltage and calculation of total charge (Q) gained during the biodegradation of organic content. Diverse samples were analyzed: acetate and peptone samples containing only soluble readily biodegradable substrates; corn starch and milk samples with suspended and colloidal organics; real domestic and brewery wastewaters. Linear regression fitted to the Q vs. BOD 5 measurement points of the real wastewaters provided high (> 0.985) R 2 values. Time requirement of the measurement varied from 1 to 4 days, depending on the composition of the sample. Conclusions Relative error of BOD measured in the MFCs comparing with BOD 5 was less than 10%, thus the method might be a good basis for the development of on-site automatic BOD sensors for real wastewater samples.

Key findings

• ▸High R² values (> 0.985) were achieved in linear regression of total charge (Q) vs. BOD5 measurement points in real wastewaters.
• ▸The measurement method consisted of batch sample injection, continuous measurement of cell voltage, and calculation of total charge (Q) gained during biodegradation.
• ▸The time requirement of the measurement varied from 1 to 4 days, depending on the composition of the sample.

Keywords

Identifiers

DOI

10.1007/s10529-020-03050-5

Journal

Biotechnology Letters

Year

2020