Analysis of denitrification performance and microbial community structure in a bio-electrochemical reactor under different current densities with wheat-rice stone powder

Abstract

Abstract Nitrate widely exists in water pollution and is the most stable form of nitrogen pollution. This study investigated the effect of the current density (CD) and the wheat-rice stone powder (WP) on denitrification performance, microbial diversity and enzyme activity in a bio-electrochemical reactor (BER). It was found that an optimum CD of 200 mA/m² and the addition of WP significantly improved the nitrate removal rate constant compared with the control group (12.28 d⁻¹ versus 9.75 d⁻¹) and remarkably reduced the intermediate accumulation of nitrite. The application of both optimum CD and WP enhanced the microbial diversity and catalytic activity of nitrate reductase (Nar) and nitrite reductase (Nir). The most dominant microbial taxa in our reactor were Methyloversatilis, Methylotenera, and an unclassified genus of the family Methylophilaceae. Moreover, WP allowed the denitrifiers to better withstand the stress of high CD. This study presented results supporting the use of an optimum CD and natural mineral addition to improving the performance of the denitrification process within a BER.

Key findings

• ▸An optimum current density of 200 mA/m² improved nitrate removal rate constant compared to the control group.
• ▸The addition of wheat-rice stone powder reduced intermediate accumulation of nitrite.
• ▸The application of both optimum current density and wheat-rice stone powder enhanced microbial diversity and catalytic activity of nitrate reductase and nitrite reductase.

Keywords

Identifiers

DOI

10.2166/wrd.2022.086

Journal

Journal of Water Reuse and Desalination

Year

2022