New Electrogenic Microorganism Citrobacter sp. Isolated from Microbial Fuel Cell and Bacterial Characteristics Determination
Hugo Guillermo Jimenez Pacheco, Abdel Alejandro Portocarrero Banda, Eric Ivan Vilca Cayllahua, Lilia Mary Miranda Ramos +3
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70% confidenceA new electrogenic microorganism, Citrobacter sp., was isolated from a microbial fuel cell and its characteristics were determined through molecular, biochemical, and phylogenetic analysis.
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Abstract
The generation of energy through the transformation of polluting waste is a widely explored field and offers advances in green technologies. One of the promising technologies is Microbial Fuel Cells (MFCs). These cells can contain electroactive microorganisms that transform organic waste into electricity by transferring electrons from their metabolism. In this study, a new bacterium capable of producing electricity from the waste of the poultry sector and using copper electrodes, called Av_G1, was identified and isolated. It is phylogenetically related to Citrobacter freundii and Citrobacter Murlinae. This new strain was identified molecularly, biochemically, and phylogenetically; its physiological and morphological characteristics were also studied through a Scanning Electron Microscope (SEM). Biochemical determination was performed using Simmons Citrate Agar, Lysine Iron Medium (L.I.A.), Motility/Ornithine Test, Methyl Red indicator, Enzymes: oxidase and catalase, and Gram stain test. The phylogenetic inference was deduced by bioinformatics tools (MEGA X, JalView, Clustal Omega) and the genetic databases The Ribosomal Database Project—RDP and the National Center for Biotechnology Information (NCBI). A maximum current potential of 0.645 V, a maximum current density of 168.72 ± 14.07 mA/m², and a power density of 31.05 mW/m² were recorded. During the monitoring, the physicochemical parameters were taken: pH, Oxide Reduction Potential (ORP), Dissolved Oxygen (DO), conductivity, Total Solids (TDS), and average temperature were recorded. Therefore, the present study shows a new Gram-negative electrogenic bacterium, which can be used for electrochemical processes and applied in MFC with copper electrodes.
Key findings
- The isolated microorganism, Av_G1, is phylogenetically related to Citrobacter freundii and Citrobacter Murlinae.
- The microorganism was identified as a Citrobacter sp. through molecular, biochemical, and phylogenetic analysis.
- The microorganism was found to be electroactive and capable of producing electricity from poultry waste using copper electrodes.
Keywords
Identifiers
- Journal
- Energies
- Year
- 2023