Microbial fuel cell type biosensor for specific volatile fatty acids using acclimated bacterial communities.

Abstract

Volatile fatty acid (VFA) concentration is one of the most important parameters for monitoring bio-processes such as anaerobic digestion and microbial fuel cells. In this study the correlation between VFA concentration and current/voltage responses and electrochemical properties by using the MFC technology was evaluated. The discrimination between different species of VFA by using two methods i.e., coulombic efficiency and cyclic voltammetry was investigated. Columbic efficiency gave a slow response of greater than 20h, particularly at concentration levels of 20mgl⁻¹. By using cyclic voltammetry to measure the oxidation peak at a consistent scan rate showed linear correlation to VFA concentration and peak current produced, up to <40mgl⁻¹) in a rapid response time of 1-2min. The results presented showed good correlations between the individual VFA species concentration and charge, and also current generated. A MFC based biosensor array was produced capable of measuring individual acetate, propionate and butyrate concentrations with sensitivity down to 5mgl⁻¹ and up to 40mgl⁻¹.

Key findings

• ▸Coulombic efficiency showed a slow response time of over 20 hours to VFA concentrations above 20 mg/L.
• ▸Cyclic voltammetry provided a rapid response time of 1-2 minutes to VFA concentrations up to 40 mg/L.
• ▸A MFC-based biosensor array was developed to measure individual acetate, propionate, and butyrate concentrations with sensitivity down to 5 mg/L.

Keywords

Identifiers

DOI

10.1016/j.bios.2013.02.033

PubMed

23545174

Journal

Biosensors &amp; bioelectronics

Year

2013