Chitin biomass powered microbial fuel cell for electricity production using halophilic Bacillus circulans BBL03 isolated from sea salt harvesting area.

Abstract

Incessant depletion of non-renewable energy sources has gained attention to search for new biological systems to transform organic biomass into electricity using microbial fuel cell (MFC). The main approach of the existing study was to develop a single step process to produce electrical energy from underutilized chitin biomass. Halophilic bacterium Bacillus circulans BBL03 isolated from anodic biofilm showed higher electricity production (26.508 μAcm2) in a natural seawater medium fed with 1.0% chitin. Maximum chitinase activity (94.24 ± 4.2 U mL-1) and N-acetylglucosamine (GlcNAc) production (136.30 ± 2.8 mg g⁻¹ chitin) were achieved at 48 h. Prominent metabolites detected in chitin hydrolysis were lactate, formate, acetate, propionate, and butyrate. Furthermore, cyclic voltammetry (CV) studies revealed the possibility of direct electron transfer by anodic biofilm to anode without any external redox mediators. Polarization and coulombic efficiency (CE) analysis showed maximum power density (PD) 1.742 mWcm2 and 47% CE using 1% chitin as a substrate. Alteration in crystallinity and functional group on chitin were analysed using FTIR and XRD. Therefore, natural seawater-chitin powered MFCs could be a cheap asset for longer electricity production.

Key findings

• ▸Halophilic bacterium Bacillus circulans BBL03 showed higher electricity production (26.508 μAcm2) in a natural seawater medium fed with 1.0% chitin.
• ▸Maximum chitinase activity (94.24±4.2 U mL-1) and N-acetylglucosamine (GlcNAc) production (136.30±2.8 mg g⁻¹ chitin) were achieved at 48 h.
• ▸Cyclic voltammetry studies revealed direct electron transfer by anodic biofilm to anode without external redox mediators.

Keywords

Identifiers

DOI

10.1016/j.bioelechem.2019.107329

PubMed

31325898

Journal

Bioelectrochemistry (Amsterdam, Netherlands)

Year

2018