Back to research
Expression and purification of ELP-intein-tagged target proteins in high cell density E. coli fermentation
Baley A Fong, David W Wood
Source PDF
Not BES
No 3D model is mapped to this paper yet. Parameter ranges above still place reported values on the literature distribution.
Abstract
Elastin-like polypeptides (ELPs) are useful tools that can be used to non-chromatographically purify proteins. When paired with self-cleaving inteins, they can be used as economical self-cleaving purification tags. However, ELPs and ELP-tagged target proteins have been traditionally expressed using highly enriched media in shake flask cultures, which are generally not amenable to scale-up.
Keywords
InteinFermentationBiochemistryYield (engineering)Escherichia coliProtein expression
Identifiers
- PubMed
- 20959011
- Journal
- Microbial Cell Factories
- Year
- 2009