MESSAI.iO

AI summary

70% confidence

The cytochrome PccH from Geobacter sulfurreducens (Gs) plays a crucial role in current‐consuming fumarate‐reducing biofilms. Deletion of pccH gene inhibited completely electron transfer from electrodes toward Gs cells.

Generated by MESSAI extraction pipeline · review against source PDF

Generic MECRepresentative model
Click to animate flow
loading 3D model…

Representative MEC — matched on the paper’s system type only, not its reactor or geometry.

Extraction

Reported parameters

1 extracted value

View extracted values

Open in lab for full controls, parameter editing, and template overlays.

Open in lab →

What they did

System
MEC

What worked

No outcome metrics extracted yet.

Abstract

The cytochrome PccH from Geobacter sulfurreducens (Gs) plays a crucial role in current‐consuming fumarate‐reducing biofilms. Deletion of pccH gene inhibited completely electron transfer from electrodes toward Gs cells. The pccH gene was cloned and the protein heterologously expressed in Escherichia coli. Complementary biophysical techniques including CD, UV–visible and NMR spectroscopy were used to characterize PccH. This cytochrome contains one low‐spin c‐type heme with His–Met axial coordination and unusual low‐reduction potential. This reduction potential is pH‐dependent, within the Gs physiological pH range, and is discussed within the context of the electron transfer mechanisms from electrodes to Gs cells.

Keywords

Geobacter sulfurreducensElectron transferCytochrome cCytochromeContext (archaeology)Geobacter

Identifiers

DOI
10.1016/j.febslet.2013.07.003
Journal
FEBS Letters
Year
2013