Microaerophilic microenvironment at biocathode enhances electrogenesis with simultaneous synthesis of polyhydroxyalkanoates (PHA) in bioelectrochemical system (BES)

Abstract

Microaerophilic microenvironment at biocathode was evaluated for electrogenesis along with the polyhydroxyalkanoates (PHA) accumulation in bio-electrochemical system (BES). The electrogenic activity (512 mV; 15.2 mW/m²) was extended for longer periods (144 h) which might be attributed to the lowering of losses due to the controlled microbial metabolism. Growth limiting stress at cathode due to lower oxygen levels and its effective utilization by the protons and electrons coming from anode, might have diverted the microbial metabolism towards PHA synthesis instead of oxidation. PHA accumulation (19% of dry cell weight (DCW)) was observed with higher hydroxy butyrate (HB) (89%) concentration at 48 th h in the cathodic biocatalyst and was re-utilized by the end of experiment. Bio-electro kinetics studied through voltammetry and Tafel analysis further supported the observed electrogenesis in microaerophilic reduction microenvironment, in terms of redox catalytic currents, Tafel slopes, exchange current densities and polarization resistance.

Keywords

Identifiers

DOI

10.1016/j.biortech.2012.08.060

PubMed

23037883

Journal

Bioresource Technology

Year

2012