Defined Electrosynthetic Microbial Consortia Reveal Electron Transfer Modes Governing Acetate Production.
AI summary
75% confidenceThe study demonstrates the importance of interspecies electron transfer in microbial electrosynthesis, achieving enhanced acetate production through defined synthetic consortia. The consortia, composed of Shewanella oneidensis MR-1 and either Clostridium aceticum or Acetobacterium woodii, exhibit distinct electron transfer modes. This approach enables efficient CO2 bioconversion, with carbon conversion efficiencies exceeding 84%.
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Abstract
Precise manipulation of interspecies electron transfer (IET) is critical for advancing microbial electrosynthesis (MES) toward efficient CO 2 bioconversion. Here, defined synthetic consortia are constructed by pairing Shewanella oneidensis MR-1, a model bidirectional electroactive bacterium, with Clostridium aceticum (electroactive acetogen) or Acetobacterium woodii (nonelectroactive acetogen), mimicking functional guilds commonly observed in natural MES communities. Co-cultivation markedly boosts acetate production by up to 88%, achieving 1.16 ± 0.01 and 1.05 ± 0.01 g L -1 , with carbon conversion efficiencies exceeding 84%. Comprehensive electrochemical, spectroscopic, and biomass analyses reveal distinct spatial electron transfer modes: DIET via cytochrome c and riboflavin dominates at the biofilm-electrode interface in S. oneidensis-C. aceticum consortia, whereas S. oneidensis-A. woodii consortia prefer H 2 /formate-mediated IET in the planktonic phase. This metabolic stratification enables S. oneidensis to function as an "ecosystem engineer," orchestrating electron flow to optimize CO 2 -to-acetate conversion across biofilm and suspension niches. The proposed synthetic ecology strategy provides a blueprint for designing high-efficiency MES consortia, paving new avenues for sustainable carbon capture and bio-based chemical production.