MESSAI.iO

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International MES research in biotechnology with focus on biotechnology. Abstract Microplastics are contaminants of global concern that pose risks to ecosystems ...

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System
not_BES

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Abstract

Abstract Microplastics are contaminants of global concern that pose risks to ecosystems and human health. Focusing on PET plastics, we present a proof-of-concept for reduction of microplastic pollution: in situ engineering of bacteria in wastewater to degrade PET. Using a broad-host-range conjugative plasmid, we enabled various bacterial species from a municipal wastewater sample to express FAST-PETase, which was released into the extracellular environment. We found that FAST-PETase purified from some isolates could degrade about 40% of a 0.25 mm thick PET film within four days at 50 °C. We then demonstrate partial degradation of post-consumer PET over 5-7 days by exposure to conditioned media from isolates. These results have broad implications for addressing the global plastic pollution problem by enabling environmental bacteria to degrade PET plastics in situ .

Keywords

WastewaterMicroplasticsDegradation (telecommunications)BacteriaPollutionContamination

Identifiers

DOI
10.1101/2024.02.07.579132
Journal
bioRxiv (Cold Spring Harbor Laboratory)
Year
2024