Evaluation of soil microbial activity after application of Paenibacillus polymyxa-based biofertilizer in wheat field experiment

Abstract

The establishment of eco-friendly principles in agriculture requires, from one side, a reduced dependence on chemical fertilizers, pesticides and herbicides, and on the other, a maintenance of consistent yield and high plant productivity. Such substantial shift in the contemporary agricultural practices can be achieved only if a wide variety of alternative options is available. Furthermore, in order to become part of agricultural practices these options have to be affordable in a broad scale and effective against the loss of biodiversity and reduced soil fertility. The current study presents data about the metabolic activity and microbial community` structure in two soil samples – one treated with chemical fertilizer and another treated with chemical fertilizer reduced by 25% dose and supplemented with commercially available biofertilizer. Metabolic activity and properties of microbial soil communities were assessed by the BIOLOG® EcoPlate technique. The estimated parameters included average well-color development (AWCD) and utilisation of six guilds of substrates. Several functional indexes that are used for evaluation of community diversity and evenness were also estimated. The AWCD, expressed either as total activity or as guilds utilisation during the EcoPlate incubation period of seven days did not reveal significant difference between variants. However, most of the estimated functional indexes consistently indicated higher microbial diversity and more balanced structure of microbial community in the biofertilizer-supplemented variant. The results clearly implied that the biofertilizer based on nitrogen-fixing bacteria can positively influence the structure of soil communities and its application could be beneficial for soil fertility and plant productivity. Keywords: diazotroph, biofertilizer, BIOLOG® EcoPlate, functional indexes, metabolic activity, microbial communities

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Identifiers

DOI

10.22620/agrisci.2024.40.003

Journal

Agricultural Sciences

Year

2024