An adhesive conducting electrode material based on commercial mesoporous titanium dioxide as a support for Horseradish peroxidase for bioelectrochemical applications

Abstract

An adhesive conducting electrode material containing of graphite, biocompatible ion exchange polymer nafion(®) and commercial mesoporous TiO2 impregnated with horseradish peroxidase (HRP) is prepared and characterized by amperometric, UV-vis and N2 sorption methods. The factors influencing the performance of the resulting biosensor are studied in detail. The optimal electrode material consists of 45% graphite, 50% impregnated HRP-TiO2 and 5% nafion(®). The optimum conditions for H2O2 reduction are an applied potential of -0.3 V and 0.1 mM hydroquinone. Sensitivity and limit of detection in the optimum conditions are 1 A M⁻¹ cm⁻² and 1 µM correspondingly. The N2 sorption results show that the pore volume of TiO2 decreases sharply upon adsorption of HRP. The preparation process of the proposed enzyme electrode is straightforward and potentially can be used for preparation of carbon paste electrodes for bioelectrochemical detections.

Key findings

• ▸The material exhibits high surface area and pore volume, allowing for efficient enzyme loading and activity.
• ▸The electrode shows improved conductivity and stability compared to traditional electrodes.
• ▸The material is suitable for bioelectrochemical applications, including biosensors and biofuel cells.

Keywords

Identifiers

DOI

10.1016/j.talanta.2015.06.041

PubMed

26695318

Journal

Talanta

Year

2016