Electrical Stimulation Improves Microbial Salinity Resistance and Organofluorine Removal in Bioelectrochemical Systems

Abstract

ABSTRACT Fed batch bioelectrochemical systems (BESs) based on electrical stimulation were used to treat p -fluoronitrobenzene ( p -FNB) wastewater at high salinities. At a NaCl concentration of 40 g/liter, p -FNB was removed 100% in 96 h in the BES, whereas in the biotic control (BC) (absence of current), p -FNB removal was only 10%. By increasing NaCl concentrations from 0 g/liter to 40 g/liter, defluorination efficiency decreased around 40% in the BES, and in the BC it was completely ceased. p -FNB was mineralized by 30% in the BES and hardly in the BC. Microorganisms were able to store 3.8 and 0.7 times more K + and Na + intracellularly in the BES than in the BC. Following the same trend, the ratio of protein to soluble polysaccharide increased from 3.1 to 7.8 as the NaCl increased from 0 to 40 g/liter. Both trends raise speculation that an electrical stimulation drives microbial preference toward K + and protein accumulation to tolerate salinity. These findings are in accordance with an enrichment of halophilic organisms in the BES. Halobacterium dominated in the BES by 56.8% at a NaCl concentration of 40 g/liter, while its abundance was found as low as 17.5% in the BC. These findings propose a new method of electrical stimulation to improve microbial salinity resistance.

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Identifiers

DOI

10.1128/aem.04066-14

Journal

Applied and Environmental Microbiology

Year

2015