Isolation, Screening and Characterization of Polyhydroxybutyrate (PHB) Producing Bacteria from Landfill Soil

Abstract

Today, environment is polluted with waste which is not degradable. Waste placed at landfill dumping site which contain plastics account about 20% by volume of municipal solid wastes and reduce the capacity of precious landfill sites. Poly hydroxyl butyrate (PHB) which is a biodegradable and biocompatible thermoplastic compound has broadly similar physical properties to poly (propylene). It has many applications in medicine, veterinary practice, tissue engineering materials, food packaging and agriculture due to its biodegradability. In the present study, we focus on screening of bioplastics producing bacterial isolates from the soil sample from dumping site. The bacteria were characterized by their cultural characteristics, morphology and biochemical test. PHB producing bacterial isolate was further detected by using Sudan black- B staining reagent. Attempt was made to produce and extract PHB by using our bacterial isolate. Isolate exhibited significant PHB yields, thus showing a potential for further exploitation. Further analyses are currently ongoing to try to extract and characterize PHB granules.

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Identifiers

DOI

10.20546/ijcmas.2023.1201.012

Journal

International Journal of Current Microbiology and Applied Sciences

Year

2023