Electrochemical Evaluation of Bacterial Activity

Abstract

For the efficient utilization of bacterial bioresources, the quantitative evaluation of metabolic activity in live bacterial cells is required. Using potentiometric measurements, we quantitatively evaluated the electron generation rate of Shewanella oneidensis MR-1 based on individual enzymatic reactions. We evaluated intracellular electron generation in bacterial suspensions supplemented with different carbon sources utilized in the tricarboxylic acid cycle. In bacterial suspensions, ferricyanide was almost completely reduced to ferrocyanide by cell-generated electrons, without an effect on bacterial cell viability. Focusing on this reduction reaction, quantitative evaluations were possible by potentiometry based on the Nernst equation.

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Identifiers

DOI

10.1149/osf.io/2q4d8

Journal

Unknown journal

Year

2020