Enhancement of Electrochemical Performance of Bilirubin Oxidase Modified Gas Diffusion Biocathode By Porphyrin Precursor

Abstract

Recent studies have focused on tailoring the catalytic currents of multicopper oxidase (MCO) enzymes-based biocathodes to enhance oxygen reduction. Biocathodes modified with natural substrates specific for MCO enzymes demonstrated drastic improvement for oxygen reduction. Performance of 1- pyrenebutanoic acid, succinimidyl ester (PBSE) and 2,5- dimethyl-1-phenyl-1H-pyrrole-3-carbaldehyde (Di-Carb) oriented bilirubin oxidase (BOx) modified gas diffusion biocathode has been drastically improved by incorporating, a porphyrin precursor as electron transfer moiety. Porphyrin precursor modified electrodes demonstrated direct electron transfer reaction of BOx exhibiting larger O 2 reduction current density in phosphate buffer solution (pH 7.0) without the need of a mediator. A remarkable improvement in the catalytic currents with 2.5 fold increase was achieved compared to non-modified oriented BOx electrodes. Moreover, a mediator-less and compartment-less Glucose/O 2 biofuel cell based on direct electron transfer (DET) type bioelectrocatalysis via the BOx-cathode and the glucose dehydrogenase (GDH)-anode demonstrated peak power densities of 1mW/cm 2 at pH 7.0 with 100mM glucose/10mM NAD fuel. The maximum current density of 1.6mA/cm 2 and the maximum power density of 0.4mW/cm 2 were achieved at 300mV with non modified BOx cathode, while 3.5mA/cm 2 and 1.1mW/cm 2 of current and power density was achieved with porphyrin precursor modified cathode. The performance improved 2.4 times which attributes to the porphyrin precursor acting as a natural substrate and activator for BOx activity enhancement.

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Identifiers

DOI

10.1149/ma2018-01/38/2245

Journal

ECS Meeting Abstracts

Year

2018