Accumulation of NH₄⁺ and NO₃⁻ inside Biofilms of Natural Microbial Consortia: Implication on Nutrients Seasonal Dynamic in Aquatic Ecosystems

Abstract

Microbial biofilms are ubiquitous in aquatic ecosystems. Inside the biofilm is the nutrient-rich microenvironment promoted by the accumulation of the nutrient ions such as NH 4 + and NO 3 - from surrounding water. The present study investigated the characteristics of NH 4 + and NO 3 - accumulation into the biofilm of natural microbial consortia collected from Lake Biwa, Japan. The results showed the following: (1) the concentrations of NH 4 + and NO 3 - inside the biofilm were much higher than those in the surrounding water; (2) the nutrient ion concentration inside the biofilm changed in synchrony with those in the surrounding water; (3) biofilm polymers have both positively and negatively charged sites; (4) electrostatic attractive interactions between the charged sites on biofilm polymers and oppositely charged ions outside the biofilm seem to play important roles in the accumulation of nutrient ions into the biofilm from the surrounding water; (5) the bacterial community structure differs between the biofilm and surrounding water. The present study revealed that the accumulation of nutrient ions into the biofilm indicates the removal of these ions from water outside the biofilm. According to the result of this study, accumulation of ions such as NH 4 + and NO 3 - into the biofilm of natural microbial consortia may have implications on nutrients seasonal dynamic in aquatic ecosystems.

Keywords

Identifiers

DOI

10.1155/2019/6473690

PubMed

31281363

Journal

International Journal of Microbiology

Year

2019