Preparation of the Proteus Mirabilis Bacterial Electrode For the Determination of Urea and It's Clinical Applications

Abstract

Abstract A bacterial electrode for the determination of urea has been constructed by immobilizing the Proteus mirabilis on a carbon dioxide gas-sensor. the electrode gave a Nernstian behaviour between 7.0 × 10⁻⁴ and 3.0 × 10⁻² M urea with a slope of 46 mV/decade in pH 6.80, 0.1M phosphate buffer at 30°C. the important interferences were L-asparagine, cytosine, inositol and phenol, and most inorganic salts reacted as the inhibitor. This electrode showed little change in the response and linear rane for 7 days, and could also be used in the linear range because the electrode had good reproducibility even after this. This device could be used as easily and exactly as a spectrophotometric method in clinical applications.

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DOI

10.1080/00032718908051181

Journal

Analytical Letters

Year

1989