Taxonomic distribution of alkane synthesizing aldehyde dehydrogenases across bacteria, fungi, plants, and animals.
AI summary
75% confidenceThis MES study investigates Aldehyde decarbonylase, Aldehyde dehydrogenase, Biofuel. The research focuses on system optimization and characterization.
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Abstract
Hydrocarbon-based biofuels, known as drop-in fuels, which are chemically similar to petroleum, have gained significant attention. Microorganisms that produce medium-chain alkanes hold promise for the bioproduction of drop-in fuels. Previous studies identified Klebsiella sp. NBRC100048 as having aldehyde-decarbonylating activity, enabling it to convert aldehydes into alkanes. Using a genomic fosmid library from Klebsiella sp. NBRC100048, we identified open reading frame 2991 (orf2991), which catalyzes the conversion of tetradecanal to tridecane. This gene shares high sequence similarity with the aldehyde dehydrogenase (ALDH) family in Escherichia coli.