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MES research in electrode, membrane. Dielectrophoresis (DEP) is one of an alternative way for cell separation. It has mainly been...

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Abstract

Dielectrophoresis (DEP) is one of an alternative way for cell separation. It has mainly been limited to processing small volumes due to constrain in fabrication of microelectrode over large surface areas. This work incorporated the wire cloth electrode fabricated using textile technology into a high throughput chamber experiment. The plain-weave wire cloth consists of 71µm stainless steel wires as the microelectrode arrays hold together by polyester yarn warp. This work determines the cell separation yield with parameters on applied voltage, flow rate and cell concentration as well as its optimized variables on the chamber width of 1.2cm and 2.5cm. The optimum voltage achieved was 30Vpk-pk, with flow rate of 3.5 ml/min and maximum cell concentration of 2.08x107 cells/ml. In chamber width comparison, 1.2cm width chamber gives better total percentage yield of 96% than the 2.5cm width chamber of 85% total percentage yield.

Keywords

ElectrodeMicroelectrodeYield (engineering)Volumetric flow rateDielectrophoresisVoltage

Identifiers

DOI
10.35940/ijitee.f1077.0486s419
Journal
International Journal of Innovative Technology and Exploring Engineering
Year
2019