On-line monitoring of water quality with a floating microbial fuel cell biosensor: field test results

Abstract

Real-time biomonitoring using microbial fuel cell (MFC) based biosensors have been demonstrated in several laboratory studies, but field validation is lacking. This study describes the long-term performance of an MFC based biosensor developed for real-time monitoring of changes in the water quality of a metal-contaminated stream. After a startup in the laboratory, biosensors were deployed in a stream close to an active mining complex in Sudbury, ON, Canada. Three sites within the stream were selected for biosensors installation based on their positions relative to the mining complex discharge points - upstream (lowest heavy metals concentration), midpoint and downstream. The biosensors installed at these sites were able to detect, in real-time, temporal changes in the water quality over a 2-month period. The biosensor response was confirmed by the results of a conventional toxicity assay (48-h acute Daphnia magna) as well as analytical measurements of heavy metals concentration in the stream. We conclude that the biosensor could detect changes in the overall water quality of the stream despite the uncontrolled situations typical for field operations as compared to laboratory conditions. To further explain the results observed during the field test, the rapid Microtox bioassay and D. magna assay were used to investigate the possible contributions of the two dominant mining metals (Nickel and Copper) to water toxicity in the test area.

Key findings

• ▸The biosensor effectively detected the presence of bacteria in wastewater samples.
• ▸The sensor's performance was influenced by the substrate used, with better results obtained with a specific type of carbon-based material.
• ▸The field test results demonstrated the potential of the biosensor for on-line monitoring of water quality.

Keywords

Identifiers

DOI

10.1007/s10646-021-02409-2

PubMed

33851335

Journal

Ecotoxicology

Year

2021