An essential role for the Hv1 voltage-gated proton channel in Pseudomonas aeruginosa corneal infection

Abstract

Assembly of NADPH oxidase 2 (NOX2) proteins in neutrophils plays an essential role in controlling microbial infections by producing high levels of reactive oxygen species (ROS). We reported that neutrophils and NOX2 are required to control P. aeruginosa in a clinically relevant murine model of blinding corneal infection. Given the published role for the voltage-gated proton channel Hv1 in sustaining NOX2 production, we examined the role of Hv1 in P. aeruginosa keratitis. Hvcn1−/− mice exhibited an impaired ability to kill bacteria that was associated with reduced neutrophil recruitment to infected corneas. Unlike earlier reports, we found that Hvcn1−/− neutrophils produce more rather than less ROS compared with control neutrophils infected with P. aeruginosa or stimulated with PMA or zymosan. Collectively, we demonstrate that Hv1 has an important role in control of bacterial growth by neutrophils in bacterial infection beyond the regulation of ROS production.

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Identifiers

DOI

10.1101/2024.07.15.603631

Journal

bioRxiv (Cold Spring Harbor Laboratory)

Year

2024