Sustained performance and microbial succession in novel artificial rumen system coupling dynamic membrane with methanogenic granules for acid absorption.
AI summary
75% confidenceThis MES study investigates Anaerobic digestion, Bioenergy recovery, Lignocellulose. The research focuses on system optimization and characterization.
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Abstract
Artificial rumen systems promise for converting lignocellulosic biomass into renewable products but face challenges in long-term operation. This study developed a novel artificial rumen system combined fermentation with acid absorption using methanogenic granules and a dynamic membrane. Over 480 days, volatile fatty acids (VFAs) were effectively separated by the dynamic membrane and immediately absorbed by granules, simulating natural ruminant acid absorption. Despite increasing the organic loading rate from 3.27 to 8.18 g-VS/L/day, stable VFA yields of 0.21-0.24 g-COD/g-VS were maintained. After 440 days, removal efficiencies for cellulose, hemicellulose, and lignin reached 62.7 %, 52.1 %, and 40.7 %, respectively. The acid absorption unit efficiently converted VFAs into biomethane (302-304 mL/g COD), showing high bioenergy potential. Metagenomic analysis confirmed key rumen microbes (Firmicutes, Bacteroidetes) were dominant, with enrichment of low-abundance species like Prevotella and Solobacterium that secreted lignocellulose-degrading enzymes. The system enables long-term biomass conversion and supports future high-load artificial rumen engineering.