Antimicrobial and Anticancer Efficacy of Atmospheric Pressure Cold Plasma Technology

Abstract

The present work evaluated the inactivation efficiency of atmospheric pressure cold plasma (APCP) against three pathogenic microorganisms. We have successfully developed a plasma device that can generate atmospheric pressure cold argon plasma of low temperature (240 - 270C) downstream using a high-voltage power source (6 kV) operating at a frequency of 19.56 kHz, which can be widely used in biomedicine. Therefore, a cost-effective system of generating cold plasma jets at atmospheric pressure with potential applications in biomedical research has been developed. The discharge has been characterized by an optical method. This research aims to investigate the antimicrobial property shown by the atmospheric pressure cold plasma Jet (APCPJ). Three pathogenic bacterial strains (Shigella flexneri, Escherichia coli, and Klebsiella pneumoniae) were used to test the antimicrobial property. The effects of atmospheric pressure cold plasma (APCP) today have been identified worldwide in disinfection, decontamination, and sterilization, as well as oncology applications. This work aims to demonstrate the effect of APCP irradiated media as a promising anticancer tool. Atmospheric pressure cold plasma technology has the efficacy to show anticancer properties with the treatment of cancer cells. To demonstrate the anticancer properties of APCP generated at a high voltage power supply (12 kV) at an operating frequency of 50 Hz. 10% Dulbecco’s Modified Eagle Medium (DMEM) media were treated with cold plasma using argon as a process gas for various time durations (0.5-4 min). The treated media was transferred to Henrietta Lacks (HeLa) and Human Embryonic Kidneys 293(HEK 293) cells, and the viability of cancer cells was observed using MTT assay.

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Identifiers

DOI

10.33263/materials41.001

Journal

Materials International

Year

2022