MESSAI.iO

AI summary

100% confidence

MES research in membrane, bacteria. The most biological diversity on this planet is probably harbored in soils. Understanding th...

Generated by MESSAI extraction pipeline · review against source PDF

Extraction

Reported parameters

1 extracted value

View extracted values

No 3D model is mapped to this paper yet. Parameter ranges above still place reported values on the literature distribution.

Abstract

The most biological diversity on this planet is probably harbored in soils. Understanding the diversity and function of the microbiological component of soil poses great challenges that are being overcome by the application of molecular biological approaches. This review covers one of many approaches being used: separation of polymerase chain reaction (PCR) amplicons using denaturing gradient gel electrophoresis (DGGE). Extraction of nucleic acids directly from soils allows the examination of a community without the limitation posed by cultivation. Polymerase chain reaction provides a means to increase the numbers of a target for its detection on gels. Using the rRNA genes as a target for PCR provides phylogenetic information on populations comprising communities. Fingerprints produced by this method have allowed spatial and temporal comparisons of soil communities within and between locations or among treatments. Numerous samples can be compared because of the rapid high throughput nature of this method. Scientists now have the means to begin addressing complex ecological questions about the spatial, temporal, and nutritional interactions faced by microbes in the soil environment.

Keywords

Temperature gradient gel electrophoresisAmpliconPolymerase chain reactionSoil waterMicrobial population biologyEnvironmental DNA

Identifiers

DOI
10.2136/sssaj2006.0080
Journal
Soil Science Society of America Journal
Year
2007