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International MES research in heritage with focus on cultural-heritage. Stabilising samples of microbial communities for DNA extraction without access to laboratory equipment can be a challeng...

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Abstract

Stabilising samples of microbial communities for DNA extraction without access to laboratory equipment can be a challenging task. In this paper we propose a method using filter paper disks for the preservation of DNA from diverse microbial communities which are found in a fermented milk product. Small adaptations to the DNA extraction method used for liquid fermented milk delivered DNA of sufficient amounts and quality to be used for later analyses, e.g. full community 16S amplicon sequencing. The microbial community structure obtained via the filter paper method showed sufficient resemblance to the structure obtain via the traditional DNA extraction from the liquid milk sample. This method can therefore successfully be used to analyse diverse microbial communities from fermented milk products from remote areas.

Keywords

DNA extractionAmplicon sequencingMicrobial population biologyFermentationMetagenomicsAmplicon

Identifiers

DOI
10.1186/s13104-019-4187-2
Journal
BMC Research Notes
Year
2019