Hyphomicrobium bacterial electrode for determination of monomethyl sulfate

Abstract

Abstract A biosensor consisting of physically entrapped monomethyl sulfate (methyl sulfate) degrading bacterium, Hyphomicrobium MS 219 , and a combined glass electrode has been developed for the determination of methyl sulfate. The response of the bacterial electrode is linear between 2.5 × 10 −2 M and 6.3 × 10 −1 M methyl sulfate with an effective response to concentrations as low as 10 −3 M and as high as 1 M methyl sulfate. The probe has an average slope of 8 mV per concentration decade over the linear range. Response times vary from 5 min in the linear range to 30 min at the detection limit. The sensor has a lifetime of at least 1 week and shows high selectivity to methyl sulfate in the absence of other growth substrates.

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Identifiers

DOI

10.1002/bit.260270623

Journal

Biotechnology and Bioengineering

Year

1985