Pyocyanin yield improvement for enhancement of Pseudomonas aeruginosa inoculated Microbial Fuel Cell efficiency

Abstract

Pseudomonas aeruginosa produces Pyocyanin which serves as a good electron transport shuttle in Microbial Fuel Cells. Studies have implicated higher Microbial Fuel Cell energy yield with higher PCN production by P. aeruginosa thus improving energy output. For obtaining high pyocyanin producing strain of P. aeruginosa, sewage water samples were taken from various sites in Islamabad and were cultured in LB media at 37°C. Characterization was performed by both biochemically as well as genetically. For pyocyanin yield assessment Rf value and λmax were determined. Pyocyanin was isolated via repeated chloroform and acidified water extraction and quantified. Physiochemical and nutritional conditions for pyocyanin production were optimized in the form of designed modified semisynthetic media. Optimized production was achieved and pyocyanin yield was increased to 3 folds than normal and control conditions. The designed pyocyanin yield media can be used to improve energy output in microbial fuel cells.

Keywords

Identifiers

DOI

10.1109/PGSRET.2018.8685940

Journal

2018 International Conference on Power Generation Systems and Renewable Energy Technologies (PGSRET)

Year

2018