An electrochemical biosensor for the detection of epithelial-mesenchymal transition

Abstract

Epithelial-mesenchymal transition (EMT) is critically involved in a variety of biological processes. Electrochemical sensing offers potential to develop more effective technology for EMT detection. In this study, by using the unique performance of quantum dot (QD)-nanocomposite materials, we establish an electrochemical biosensor that can specifically detect the change of E-cadherin and analyze different stages of EMT. The signal for EMT is largely magnified due to the transmission of molecular information to the electronic device. In addition, differential pulse voltammetry reveals that the response of the electrochemical signals is rapid and sensitive, due to the synergistic effect of QDs and carbon nanotube-gold nanoparticles. Our study thus suggests that electrochemical sensing is an effective technology for detecting EMT and may have broad applications in analyzing various cell type transitions. Epithelial-mesenchymal transition (EMT) plays a key role in embryonic development, wound healing and cancer. Here the authors develop an electrochemical sensor to detect EMT using E-cadherin antibody-quantum dot conjugates and a carbon nanotube-gold nanoparticle-modified electrode as a detection platform.

Keywords

Identifiers

DOI

10.1038/s41467-019-14037-w

Journal

Nature Communications

Year

2020