Discover insights from thousands of peer-reviewed papers on microbial electrochemical systems
A. Ruff, F. Conzuelo, W. Schuhmann
Nature Catalysis · 2019
Bioelectrocatalysis provides access to sustainable and highly efficient technological applications. However, several limitations related either to the intrinsic properties of the biocatalyst or to technical difficulties still hamper or even prevent the integration of such devices into technologically relevant large-scale processes. In this Review, we challenge the common viewpoint suggesting biology-based catalytic systems as a promising approach for the provision of sustainable stored energy and discuss the status of bioelectrocatalytic devices developed for energy conversion. In particular, we focus on two major research areas in the field, that is, H 2 -powered hydrogenase-based biofuel cells and biophotoelectrodes for solar energy harvesting. We identify the main limitations that have to be addressed to gain access to applied large-scale bio-based and bio-inspired advanced energy conversion systems. Moreover, we show recent examples and milestones that are paving the way towards potential realization of these technologies by overcoming existing limiting factors. Bioelectrocatalysis provides access to sustainable and highly efficient technological applications, but several limitations still prevent the large-scale integration of such devices. This Review discusses the current status of hydrogenase-based biofuel cells and biophotoelectrodes for solar energy harvesting.
Zhongjie Han, Fei Wu, Ping Yu et al.
Analytical Chemistry · 2022
Highly π-conjugated (hetero)cyclic molecules having delocalized orbitals and tunable charge mobilities are attractive redox relays for mediated bioelectrocatalysis in manifold applications. As rigid molecules, their dynamics within the soft but confined intraprotein space becomes the crucial determinant of the enzyme-mediator electron-tunneling efficiency. However, it is rarely investigated in designing the mediated interface with a particular biocatalyst (e.g., oxidoreductase), which remains an empirical but try-and-error process. Herein, we present the computer-aided exploration of interactions between a flavin-containing reductive synthase and structurally diverse π-extended (hetero)cyclic mediators to realize reversed bioelectrocatalytic oxidation at low overpotentials. Compared to ring-fused systems with unbroken molecular planarity, heteroatom-bridged cyclic, and rotatable conjugated structures (e.g., indophenols) can experience unusually large dynamic torsion under biased forces of hydrogen bonding with enzyme residues. This behavior led to fast intraprotein reorientation (<50 ps) that shortened the electron-tunneling distance from 12 to 9 Å. Meanwhile, the lowest unoccupied molecular orbital level upon molecular torsion was decreased by 0.5 eV to further promote electron abstraction from the reduced flavin cofactor. An efficient distant electron tunneling also obviated mediator transport through the substrate channel, thus avoiding competitive inhibition on enzyme kinetics to broaden the operating concentration range. The resulting bioelectrocatalytic interface enables low-potential biosensing of glutamate with improved selectivity. Our finding provides new structural insights into constructing efficient long-range heterogeneous charge transport with biomacromolecular catalysts.
Shize Zheng, Chenxi Zhang, P. Zhan et al.
Green Chemistry · 2025
The valorization of biomass derivatives into fine chemicals through the electro-enzymatic catalysis combination routes under green chemistry scopes has a promising prospect. However, bottlenecks including poor electron transfer efficiency between...
Tatsushi Yoshikawa, F. Makino, T. Miyata et al.
Chemical Communications · 2022
Tungsten-containing formate dehydrogenase from Methylorubrum extroquens AM1 (FoDH1)-a promising biocatalyst for the interconversion of carbon dioxide/formate and nicotine adenine dinucleotide (NAD+)/NADH redox couples-was investigated using structural biology and bioelectrochemistry. FoDH1 is reported to be an enzyme that can realize "direct electron transfer (DET)-type bioelectrocatalysis." However, its 3-D structure, electrode-active sites, and electron transfer (ET) pathways remain unclear. The ET pathways were investigated using structural information, electrostatic interactions between the electrode and the enzyme, and the differences in the substrates. Two electrode-active sites and multiple ET pathways in FoDH1 were discovered.
Lucia S. Ferraraccio, Donatella Di Lisa, L. Pastorino et al.
Analytical Chemistry · 2022
A simple procedure to incorporate enzymes (horseradish peroxidase, HRP, and lactate oxidase, LOx) within alginate hydrogels is reported with electrochemiluminescence (ECL) used to detect the enzymatic reactions with the corresponding substrates. First, HRP and LOx were successfully immobilized into CaCO3 microspheres, followed by the electrostatic layer-by-layer deposition of a nanoshell onto the microspheres, and finally by their dispersion into alginate solution. The as-prepared dispersion was drop cast onto the glassy carbon electrodes and cross-linked by the external and internal gelation methods using Ca2+ cations. The enzymes encapsulated within the alginate hydrogels were characterized using cyclic voltammetry and kinetic studies performed using ECL. The results showed that the enzymatic activity was significantly maintained as a result of the immobilization, with values of the apparent Michaelis–Menten constants estimated as 7.71 ± 0.62 and 8.41 ± 0.43 μM, for HRP and LOx, respectively. The proposed biosensors showed good stability and repeatability with an estimated limit of detection of 5.38 ± 0.05 and 0.50 ± 0.03 μM for hydrogen peroxide and lactic acid, respectively. The as-prepared enzymes encapsulated within the alginate hydrogels showed good stability up to 28 days from their preparation. The sensitivity and selectivity of the enzymes encapsulated within the alginate hydrogels were tested in real matrices (HRP, hydrogen peroxide, in contact lens solution; LOx, lactic acid in artificial sweat) showing the sensitivity of the ECL detection methods for the detection of hydrogen peroxide and lactic acid in real samples.
Wenjing Lian, Xinyu Zhang, Yongbin Han et al.
Polymers · 2025
The highly selective and sensitive determination of pesticide residues in food is critical for human health protection. Herein, the specific selectivity of molecularly imprinted polymers (MIPs) was proposed to construct an electrochemical sensor for the detection of carbendazim (CBD), one of the famous broad-spectrum fungicides, by combining with the synergistic effect of bioelectrocatalysis and nanocomposites. Gold nanoparticle-reduced graphene oxide (AuNP-rGO) composites were electrodeposited on a polished glassy carbon electrode (GCE). Then the MIP films were electropolymerized on the surface of the nanolayer using CBD as the template molecule and o-phenylenediamine (OPD) as the monomer. The detection sensitivity of CBD on the heterogeneous structure films was greatly amplified by AuNP-rGO composites and the bioelectrochemical oxidation of glucose, which was catalyzed by glucose oxidase (GOD) with the help of mediator in the underlying solution. The developed sensor showed high selectivity, good reproducibility, and excellent stability towards CBD with the linear range from 2.0 × 10−9 to 7.0 × 10−5 M, and the limit of detection (LOD) of 0.68 nM (S/N = 3). The expected system would provide a new idea for the development of simple and sensitive molecularly imprinted electrochemical sensors (MIESs).
Hyeryeong Lee, S. Reginald, I. Chang
Advanced Materials Technologies · 2023
Due to the outstanding attributes of oxidoreductases, they have been utilized as biomaterials for bioelectrocatalytic systems. Herein, a simple and versatile biosynthetic approach that can designate binding position of enzymes on electrode with their surface‐orientation is suggested. In this regard, material‐selective properties of gold‐binding peptide (GBP) are exploited and genetically fused GBP to enzyme. To optimize the design of synthetic enzyme, a variable repeat number of GBP are fused to flavin adenine dinucleotide‐dependent glucose dehydrogenase gamma‐alpha complex (GDHγα) and their catalytic and gold‐binding activities are determined. The substrate catalysis and direct electrocatalytic capability of selected construct, GDHγα with three GBP repeats (GDHγα‐3GBP), are investigated on electrode. In an inorganic‐binding characterization, GDHγα‐3GBP exhibits fourfold higher affinity on gold (Au) surface and 215‐fold lower binding affinity for silicon dioxide (SiO2) than wild‐type GDHγα. Utilizing those regioselective features, fusion GDHγα is incorporated into nanotemplates comprising Au nanopatterns and SiO2 background. Thereby, nanoscale patterned GDHγα‐3GBP molecules are successfully obtained with their binding locations controlled specifically by Au nanopatterns, not SiO2. The results reveal that genetic SBP fusions enable highly selective template‐based surface assembly of biomolecules with electrically intimate cofactor‐surface interfaces. The proposed technology has remarkable potential to fabricate small‐scale biochips applied for enzyme‐based bioelectronics.
A. Abdullatypov, P. Oskin, V. Fedina et al.
Nanomaterials · 2023
This study was carried out in order to assess several modifications of carbon nanotube-based nanomaterials for their applications in laccase electrodes and model biofuel cells. The modified MWCNTs served as adapters for the immobilization of laccase from Catenuloplanes japonicus VKM Ac-875 on the surface of electrodes made of graphite rods and graphite paste. The electrochemical properties of the electrodes were tested in linear and cyclic voltammetrical measurements for the determination of the redox potential of the enzyme and achievable current densities. The redox potential of the enzyme was above 500 mV versus NHE, while the highest current densities reached hundreds of µA/cm2. Model biofuel cells on the base of the laccase cathodes had maximal power values from 0.4 to 2 µW. The possibility of practical application of such BFCs was discussed.
Jianqi Ye, Jinhua Lu, D. Wen
Materials Chemistry Frontiers · 2023
The growing energy demands and the boost in portable or implantable electronic devices have initiated substantial interest in enzymatic biofuel cells (EBFCs). Exploiting novel electrode materials is essential in promoting...
M. Fadeev, Gilad Davidson‐Rozenfeld, Zhenzhen Li et al.
ACS Applied Materials & Interfaces · 2023
The assembly of enzyme [glucose oxidase (GOx)]-loaded stimuli-responsive DNA-based hydrogels on electrode surfaces, and the triggered control over the stiffness of the hydrogels, provides a means to switch the bioelectrocatalytic functions of the hydrogels. One system includes the assembly of GOx-loaded, pH-responsive, hydrogel matrices cross-linked by two cooperative nucleic acid motives comprising permanent duplex nucleic acids and “caged” i-motif pH-responsive duplexes. Bioelectrocatalyzed oxidation of glucose leads to the formation of gluconic acid that acidifies the hydrogel resulting in the separation of the i-motif constituents and lowering the hydrogel stiffness. Loading of the hydrogel matrices with insulin results in the potential-triggered, glucose concentration-controlled, switchable release of insulin from the hydrogel-modified electrodes. The switchable bioelectrocatalyzed release of insulin is demonstrated in the presence of ferrocenemethanol as a diffusional electron mediator or by applying an electrically wired integrated matrix that includes ferrocenyl-modified GOx embedded in the hydrogel. The second GOx-loaded, stimuli-responsive, DNA-based hydrogel matrix associated with the electrode includes a polyacrylamide hydrogel cooperatively cross-linked by duplex nucleic acids and “caged” G-quadruplex-responsive duplexes. The hydrogel matrix undergoes K+-ions/crown ether-triggered stiffness changes by the cyclic K+-ion-stimulated formation of G-quadruplexes (lower stiffness) and the crown ether-induced separation of the G-quadruplexes (higher stiffness). The hydrogel matrices demonstrate switchable bioelectrocatalytic functions guided by the stiffness properties of the hydrogels.
Jiamin Huang, Yang Gao, Yanhong Chang et al.
Advanced Science · 2023
At present, the global energy crisis and environmental pollution coexist, and the demand for sustainable clean energy has been highly concerned. Bioelectrocatalysis that combines the benefits of biocatalysis and electrocatalysis produces high‐value chemicals, clean biofuel, and biodegradable new materials. It has been applied in biosensors, biofuel cells, and bioelectrosynthesis. However, there are certain flaws in the application process of bioelectrocatalysis, such as low accuracy/efficiency, poor stability, and limited experimental conditions. These issues can possibly be solved using machine learning (ML) in recent reports although the combination of them is still not mature. To summarize the progress of ML in bioelectrocatalysis, this paper first introduces the modeling process of ML, then focuses on the reports of ML in bioelectrocatalysis, and ultimately makes a summary and outlook about current issues and future directions. It is believed that there is plenty of scope for this interdisciplinary research direction.
Hui Chen, Olja Simoska, Koun Lim et al.
Chemical Reviews · 2020
Bioelectrocatalysis is an interdisciplinary research field combining biocatalysis and electrocatalysis via the utilization of materials derived from biological systems as catalysts to catalyze the redox reactions occurring at an electrode. Bioelectrocatalysis synergistically couples the merits of both biocatalysis and electrocatalysis. The advantages of biocatalysis include high activity, high selectivity, wide substrate scope, and mild reaction conditions. The advantages of electrocatalysis include the possible utilization of renewable electricity as an electron source and high energy conversion efficiency. These properties are integrated to achieve selective biosensing, efficient energy conversion, and the production of diverse products. This review seeks to systematically and comprehensively detail the fundamentals, analyze the existing problems, summarize the development status and applications, and look toward the future development directions of bioelectrocatalysis. First, the structure, function, and modification of bioelectrocatalysts are discussed. Second, the essentials of bioelectrocatalytic systems, including electron transfer mechanisms, electrode materials, and reaction medium, are described. Third, the application of bioelectrocatalysis in the fields of biosensors, fuel cells, solar cells, catalytic mechanism studies, and bioelectrosyntheses of high-value chemicals are systematically summarized. Finally, future developments and a perspective on bioelectrocatalysis are suggested.
Aiko Kurimoto, S. A. Nasseri, Camden Hunt et al.
Nature Communications · 2023
Enzyme catalysis is used to generate approximately 50,000 tons of value-added chemical products per year. Nearly a quarter of this production requires a stoichiometric cofactor such as NAD^+/NADH. Given that NADH is expensive, it would be beneficial to regenerate it in a way that does not interfere with the enzymatic reaction. Water electrolysis could provide the proton and electron equivalent necessary to electrocatalytically convert NAD^+ to NADH. However, this form of electrocatalytic NADH regeneration is challenged by the formation of inactive NAD_2 dimers, the use of high overpotentials or mediators, and the long-term electrochemical instability of the enzyme during electrolysis. Here, we show a means of overcoming these challenges by using a bioelectrocatalytic palladium membrane reactor for electrochemical NADH regeneration from NAD^+. This achievement is possible because the membrane reactor regenerates NADH through reaction of hydride with NAD^+ in a compartment separated from the electrolysis compartment by a hydrogen-permselective Pd membrane. This separation of the enzymatic and electrolytic processes bypasses radical-induced NAD^+ degradation and enables the operator to optimize conditions for the enzymatic reaction independent of the water electrolysis. This architecture, which mechanistic studies reveal utilizes hydride sourced from water, provides an opportunity for enzyme catalysis to be driven by clean electricity where the major waste product is oxygen gas. Enzymatic catalysis requires cofactors such as NAD(P)H, whose regeneration is currently accomplished via secondary enzymes or electrolytic cell. Here, the authors report an electrochemical method of cofactors regeneration without supporting enzymes or mediators, nor formation of NAD_2 dimers.
Xiaohai Wang, Zhuanzhuan Shi, Zhikai Wang et al.
Materials · 2024
The microbial hybrid system modified by magnetic nanomaterials can enhance the interfacial electron transfer and energy conversion under the stimulation of a magnetic field. However, the bioelectrocatalytic performance of a hybrid system still needs to be improved, and the mechanism of magnetic field-induced bioelectrocatalytic enhancements is still unclear. In this work, γ-Fe2O3 magnetic nanoparticles were coated on a Shewanella putrefaciens CN32 cell surface and followed by placing in an electromagnetic field. The results showed that the electromagnetic field can greatly boost the extracellular electron transfer, and the oxidation peak current of CN32@γ-Fe2O3 increased to 2.24 times under an electromagnetic field. The enhancement mechanism is mainly due to the fact that the surface modified microorganism provides an elevated contact area for the high microbial catalytic activity of the outer cell membrane’s cytochrome, while the magnetic nanoparticles provide a networked interface between the cytoplasm and the outer membrane for boosting the fast multidimensional electron transport path in the magnetic field. This work sheds fresh scientific light on the rational design of magnetic-field-coupled electroactive microorganisms and the fundamentals of an optimal interfacial structure for a fast electron transfer process toward an efficient bioenergy conversion.
Yonatan Chemla, Federico Kaufman, M. Amiram et al.
Chemical Reviews · 2024
Genetic code expansion is a promising genetic engineering technology that incorporates noncanonical amino acids into proteins alongside the natural set of 20 amino acids. This enables the precise encoding of non-natural chemical groups in proteins. This review focuses on the applications of genetic code expansion in bioelectrocatalysis and biomaterials. In bioelectrocatalysis, this technique enhances the efficiency and selectivity of bioelectrocatalysts for use in sensors, biofuel cells, and enzymatic electrodes. In biomaterials, incorporating non-natural chemical groups into protein-based polymers facilitates the modification, fine-tuning, or the engineering of new biomaterial properties. The review provides an overview of relevant technologies, discusses applications, and highlights achievements, challenges, and prospects in these fields.
Elena E. Ferapontova
Electroanalysis · 2004
Abstract The role of the electrode material in the efficiency of direct (non‐mediated) bioelectrocatalytic reduction of H 2 O 2 catalyzed by horseradish peroxidase (HRP) is studied and discussed. The variations in direct peroxidase bioelectrocatalysis when coming from carbon/graphite to metal electrodes and oxides, as well as modified electrodes, are analyzed regarding the variations in adsorption/orientation of peroxidase at the electrodes, interfacial electron transfer rates and mechanism of catalysis.
Pengbo Wan, Xiaodong Chen
ChemElectroChem · 2014
Abstract Inspired by natural biochemical promotion and inhibition of electron‐transport processes in response to real‐life physical/chemical stimuli, artificial signal‐triggered bioelectrocatalysis and modulation of the electron‐transfer processes of redox biomolecules are vitally important for understanding electron‐transport pathways in bioelectrochemical systems and for mimicking the dynamic properties of sensitive biochemical reactions in real bioprocesses. Recently, the reversible activation and deactivation of bioelectrocatalysis by external stimuli on functional electrodes integrated with redox enzymes has been established, especially at stimuli‐responsive supramolecular interfaces. Potential applications in various research fields include controllable biofuel cells, bioelectronic devices, stimuli‐responsive biosensors, energy transduction, information storage, and data processing. This Minireview aims to summarize the current state‐of‐the‐art knowledge on various controllable bioelectrocatalysts from diverse functional interfaces formed by supramolecular interactions and supramolecular assemblies. The role of the assembled interface is highlighted, and the electrochemical kinetics during “on” and “off” states of bioelectrocatalysis is discussed. Finally, possible strategies for the future design of stimuli‐responsive bioelectrocatalysts integrated with multifunctional supramolecular interfaces are presented.
Kenji Kano
Bioscience, Biotechnology, and Biochemistry · 2022
ABSTRACT Redox enzymes can work as efficient electrocatalysts. The coupling of redox enzymatic reactions with electrode reactions is called enzymatic bioelectrocatalysis, which imparts high reaction specificity to electrode reactions with nonspecific characteristics. The key factors required for bioelectrocatalysis are hydride ion/electron transfer characteristics and low specificity for either substrate in redox enzymes. Several theoretical features of steady-state responses are introduced to understand bioelectrocatalysis and to extend the performance of bioelectrocatalytic systems. Applications of the coupling concept to bioelectrochemical devices are also summarized with emphasis on the achievements recorded in the research group of the author.
Taiki Adachi, Yuki Kitazumi, Osamu Shirai et al.
Preprints.org · 2020
Direct electron transfer (DET)-type bioelectrocatalysis, which couples electrode reactions and catalytic functions of redox enzymes without any redox mediator, is one of the most intriguing subjects studied over the past decades in the field of bioelectrochemistry. In order to realize the DET-type bioelectrocatalysis and to improve the performance, nanostructures of the electrode surface have to be carefully tuned for each enzyme. In addition, enzymes can also be tuned by protein engineering approach for the DET-type reaction. This review summarizes the resent progresses in this field of the research, while taking into consideration of the importance of nanostructure of electrodes as well as redox enzymes. Described are basic concepts and theoretical aspects of DET-type bioelectrocatalysis, significance of nanostructures as scaffolds for DET-type reactions, protein engineering approached for DET-type reactions, and concepts and facts of bidirectional DET-type reactions, from a cross-disciplinary viewpoint.
Taiki Adachi, Yuki Kitazumi, Osamu Shirai et al.
Preprints.org · 2020
Bioelectrocatalysis has become one of important research fields in electrochemistry and provided a firm base for an important technology for application to various bioelectrochemical devices such as biosensors, biofuel cells, and biosupercapacitors. The understanding and technology in bioelectrocatalysis have been greatly improved by introducing nanostructured electrode materials and protein-engineering methods over the last few decades. Recently, the electro-enzymatic production of renewable energy resources and useful organic compounds (bioelectrosynthesis) also attracts worldwide attention. In this review, we summarize recent progress in applications of enzymatic bioelectrocatalysis.
Taiki Adachi, Yuki Kitazumi, Osamu Shirai et al.
Preprints.org · 2020
Bioelectrocatalysis provides the intrinsic catalytic-functions of redox enzymes to non-specific electrode reactions and is the most important and basic concept for biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multi-analyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination.
Elisabeth Lojou, Xinxin Xiao
Catalysts · 2021
Enzymatic bioelectrocatalysis relies on immobilizing oxidoreductases on electrode surfaces, leading to different applications, such as biosensors [...]
J. Connolly
JAMA: The Journal of the American Medical Association · 1982
This book represents the authors' provocative insights from decades of research into bioelectric effects. It begins with a historical but lively account of past research seeking to discover the vital animal or electrical forces distinguishing the living from the nonliving. Galvani's erroneous conclusions regarding animal electricity in 1786 were opportunistically diverted by Volta and others from the biologic realm into the technology of generating electricity. The discovery of voltaic electricity led to the battery-operated telegraph, the arc light, and, ultimately, to the major dominance of electricity in today's society. The difficult questions pertaining to electricity's role in complex living forms remained unanswered. Biochemical explanations of biologic control systems dominated. Any suggestion of electrical control systems within the body was promptly equated with "vital force" research and dismissed as unscientific. Current concepts of electrobiologic controls owe more to developments in solid state physics than to biologic research. Body functions for which
A. Rusanov, I. Roy, O. Rusanova
Physiotherapy Quarterly · 2019
Introduction. Prognostic indices of orthopaedic implications development were investigated among patients with anterior cruciate ligament (ACL) injuries on the basis of stabilography research during the functional period of rehabilitation. Methods. The study included 52 randomly selected individuals with a complete ACL tear qualified for surgical reconstruction. The patients’ age was 18–59 (average: 37.8 ± 2.0) years. All participants underwent rehabilitation treatment in the institute of Traumatology and orthopaedics, National Academy of Medical Sciences of Ukraine, and had surgeries in the institute’s clinics. The crural part of the graft was fixed with the assistance of either the RigidFix system or the Cross-Pin system, and the Biointrafix or Biosure Sync system was applied for the tibial part. Results and conclusions. on the basis of the performed analysis of indices that characterize the functional condition of the injured limb of patients with an injury of ACL of knee joint at the functional stage of rehabilitation treatment, it is possible to identify a high and medium level of loading asymmetry in the lower extremities, decrease of electrobiological activity and muscle resistance ability during bending, stretching, adduction, and abduction, as the main prognostic criterion of orthopaedic complications development.
Wei Song, Huanhuan Li, Ting Guo et al.
Suicide and Life-Threatening Behavior · 2018
OBJECTIVE The present study aimed to explore the electrophysiological correlates involved in three-dimensional psychological pain and their relationship with suicide in patients with major depressive disorder. METHOD The sample comprised 23 and nine patients with major depressive disorder with high and low risk for suicide, respectively, and 24 healthy controls. All participants completed the measurements and performed an event-related potential-based analogue of the affective incentive delay task. The event-related potential components previously associated with motivationally salient cue (contingent negative variation, P2, and cue-P3), target (target-P3), and feedback (reward vs. punishment, feedback-related negativity, and feedback-P3) stimuli were examined. RESULTS All inventory scores differed significantly among the high-risk, low-risk, and healthy control groups. During the expectant phase, the main effect of group and interaction between group and condition was significant in the average amplitudes of the cue-P2 component. During the feedback phase, the feedback-P3 elicited by positive feedback had a significant main effect of group and of the interaction between group and condition. Specifically, the feedback-P3 elicited by negative feedback in the punitive condition showed significant positive correlations with the total and subscale scores on the Three-Dimensional Psychological Pain Scale. CONCLUSIONS Feedback-P3 may be an electrobiological component underlying the processing of psychological pain in suicidality.
Yunyun Huang, Jiaxuan Liang, Haotian Wu et al.
Light: Science & Applications · 2025
Local microcurrent monitoring is of great significance for biological and battery systems, yet it poses a formidable challenge. The current measurement techniques rely on electromagnetic materials which inevitably introduce interference to the system under examination. To address this issue, a promising approach based on a dielectric fiber-optic sensor is demonstrated. The microfiber is capable of detecting microcurrent through monitoring the localized proton concentration signal with a pH resolution of 0.0052 pH units. By sensing the refractive index variation surrounding the sensor induced by the interaction between local proton concentration changes and oxidizer-treated microfiber surface through the evanescent field, this sensing mechanism effectively avoids the interference of the electromagnetic material on the performance of the tested system. This sensor exhibits a limit of detection for microcurrent of 1 μA. The sensing region is a microfiber with a diameter of 8.8 μm. It can get invaluable information that cannot be obtained through conventional electrochemical methods. Examples include photocurrent attenuation in photogenerated carrier materials during illumination, electrical activation in nerve cells, and fluctuations in the efficiency of electrical energy generation during battery discharge. This approach provides a powerful complement to electrochemical methods for the elucidation of microscale reaction mechanisms. The information provided by the prepared dielectric fiber-optic sensor will shed more light on proton kinetics and electrochemical and electrobiological mechanisms, which may fill an important gap in the current bioelectricity and battery monitoring methods.
G. Stanciu, M. Musteață, M. Armaşu et al.
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine · 2015
Epilepsy is a chronic brain disease, of varied etiology, defined by the presence of the seizures of definite epileptic nature and by evolutional criteria, made of their tendencies to repeat in absence of triggering factors, known at variable intervals. The diagnosis of this disease is based on the clinical features and electrophysiology of the brain. Electroencephalogram (EEG) is the efficiently electrobiological test assessing the impact of epilepsy on activity of the brain.The aims of this study are to describe the interictal, intraictal and postictal parameters, and to evaluate the clinical usefulness of the EEG recording in dogs with idiopathic epilepsy, using induced sleep as activation method.EEG was performed on 27 dogs with idiopathic epilepsy. Electrical potentials acquisition was performed using the electroencephalograph Neurofax S, MEB 9400K Nihon Kohden. Before the test, all dogs were sedated with medetomidine hydrochloride 30 I¼g/kg inj. i.m. Stainless steel needle electrodes were subcutaneously placed, in an 8 channel bipolar montage, according to the model of Redding and Knecht (1984).The visual and quantitative analysis of the electroencephalographic tracks in idiopathic epilepsy revealed a background activity with a high instability and diversity of aspects, as there was more discordance between the electrical and clinical findings of the epilepsy. During interictal period, in incipient cases and onset of epilepsy, the EEG alterations were discrete, resuming to a couple of overvaulted peaks and ample lent theta waves on a normal background track. When epilepsy had a longer evolution, the background activity showed an intersection of slow waves with abnormally frequent waves, rich in epilpeptiform interictal discharges like: fast spike, slow waves, poly-spike and typical or atypical spike-wave complexes. The intraictal period was characterized by electrical crisis, suddenly appeared on all derivations, then intensified by neuronal recruiting phenomenon and in 2-3 seconds the EEG anomalies spread in all brain areas, as epilpeptiform discharges became bilateral synchronous. Postictal EEG was characterized by a much flattened aspect of the tracks, almost isoelectric.In conclusion, EEG gives valuable information about parameters and the severity of changes induced by epilepsy. EEG recorded using as an induced sleep activation method is the main way which proves the presence of an epileptic focus in the absence of clinical sings.
M. Fomina, Фомина Мария Юрьевна, T. V. Melashenko et al.
· 2018
This article presents the results of clinical, neuroimaging and electrophysiological examination of newborns with cerebral ischemia, accompanied by neonatal seizures. Neonatal seizures are an early clinical manifestation of cerebral disorders in term infants. The main method for diagnosing these conditions is to record the electrobiological activity of the brain during the interictal period and to identification of patterns of epileptic seizures. The purpose of this work was to study the clinical and electrophysiological features of neonatal seizures. In the Department of Intensive care unit and the Department of Pathology of the newborn of perinatal center of the Saint Petersburg State Pediatric Medical University, we examined 21 newborns with cerebral ischemia and neonatal seizures. All children underwent a clinical and laboratory examination, neuroimaging (neurosonography and magnetic resonance imaging of the brain), electrophysiological stu dies (EEG). It was shown that in newborn infants with convulsive syndrome in the early and neonatal period of the ictal and interictal electroencephalogram, such patterns as basal rhythm depression, discontinuity of the main electrobiological activity, acute focal or multifocal activity are recorded. The author gives his own observations of the EEG recordings in children during the fetal period, represented by focal monorhythmic patterns and focal rhythmic activity in the form of acute-slow wave complexes with subsequent suppression of the main rhythm.
Д. В. Войцеховский, Д. А. Аверьянов, А. В. Щеголев et al.
Messenger of Anesthesiology and Resuscitation · 2018
The profound deepening of medicamentous sleep down to the burst-suppression electroencephalography pattern is used to provide medication-based protection of brain during preventive temporary clipping of the major arteries when performing surgery due to cerebral aneurysms. There is no consensus about the effect of profound suppression of electrobiological activity on the development of post-operative cognitive dysfunction. The goal: to evaluate the impact of anesthesia with the burst-suppression electroencephalography pattern on the post-operative cognitive status of the patients with no cerebral disorders. Subjects and methods. 30 patients were enrolled into the prospective randomized study, they all had surgeries due to degenerative spinal diseases. All patients were divided into two groups. Anesthesia in the main group (Group 1) differed from the one in the control group (Group 2); it included administration of propofol till achieving suppression of the electrobiological activity of burst-suppression electroencephalography pattern during 15 minutes. Prior to the surgery and in 4 days after it, all patients had neuro-psychological tests using Montreal Cognitive Assessment (MoCA), Frontal Assessment Battery (FAB) and numbers memorization techniques (NMT). Results. When testing in 4 days after surgery, results in the patients from Group 1 did not differ from pre-operative results of MoCA (Me before = 28, Me after = 28, Z = 0.714, p = 0.476), FAB (Me before = 18, Me after = 18, Z = 0.592, p = 0.554), memorization of numbers in the direct order (Me before = 18, Me after = 18, Z = 0.178, p = 0.859) and in the reverse order (Me before = 18, Me after = 18, Z = 0.548, p = 0.583). The results of the post-operative testing in Group 2 were compatible with pre-operative results of (Me before = 18, Me after = 18, Z = 0.459, p = 0.646), FAB (Me before = 18, Me after = 18, Z = 1.348, p = 0.178), memorization of numbers in the direct order (Me before = 18, Me after = 18, Z = 0.21, p = 0.843) and in the reverse order (Me before = 18, Me after = 18, Z = 0.809, p = 0.418). None of the tests detected significant differences between the Groups (U = 88, p = 0.319, Z = 0.995 for MoCA; U = 102.5, p = 0.644, Z = 0.394 for FAB; U = 92.0, p = 0.407, Z = -0.829 for memorization of numbers in the direct order, and U = 33.5, p = 0.62, Z = 0.572 for memorization of numbers in the reverse order). Conclusion. Anesthesia with burst-suppression electroencephalography pattern as a model of medication-based cerebral protection during temporary clipping of the major arteries does not cause the deterioration of cognitive status in the patients who had no cerebral pathology initially.
В. Н. Казайкин, В. О. Пономарев, Алексей Владимирович Лизунов et al.
Ophthalmology in Russia · 2020
Electrophysiological research today remains an important method for the objective assessment of the functional state of the components of the visual analyzer. There are methods, for example, OCT, that can objectively assess the structural and anatomical integrity of the retina, however, only indirectly shows functional activity. The undoubted advantage of EFR is the ability, excluding the subjectivity of the subject, to perform a functional topographic assessment of the malfunction of all systems of the visual analyzer. Private electrophysiology of the vision organ is represented by a variety of methods for recording the electrobiological activity of the cells of the visual analyzer: ERG, EOG, VEP, mfERG (multifocal electroretinography), and each option is directed to a separate part of it, therefore, to complete the picture, in particular in research works on animal models may use several techniques. In general, the limitation of EFR is its complexity and many confounding factors that can affect the result, ranging from stimulation parameters to the state of the patient himself. At the same time, the main area of prospective use of electrophysiological research is differential diagnosis, preclinical toxicology and scientific and experimental models. However, in recent decades, the active introduction of registration methods, including the appearance of multifocal electroretinography, as well as changes in the conditions of electrophysiological studies, open up new possibilities for the future evolution of the method. Classical methods for evaluating EFR data, taking into account the growth of data flow, do not provide detailed qualitative and quantitative information about the state of the visual analyzer. This leaves the possibility and the need for the study, optimization and algorithmization of the assessment data of the differentiated criteria inherent for a particular ocular pathology. EFRs require simpler and more adapted protocols for clinical practice, allowing a strictly differentiated approach to the smallest anatomical and functional changes, based on open databases and modern adaptation based on artificial intelligence.
Horatiu Roibu, Lidia-Cristina Bazavan, Ş. Cismaru et al.
2022 11th International Conference on Modern Circuits and Systems Technologies (MOCAST) · 2022
The ways in which humans and machines interact have changed as new technologies have been introduced into practice. At this moment, the trend is to simplify the interaction between man and machine, making the interfaces as intuitive and easy to use as possible. In this purpose, human-machine interfaces based on voice commands, gestures and even interfaces based on electrobiological signals generated by human body have been successfully developed. In this paper, the authors show the current stage, steps and experiments performed to develop a human-machine interface based on the electromyography technique. The proposed procedure involves the collection of electrical signals generated by muscles and their transformation into usable signals for the control of a robot by processing them. The interface was connected with the Kuka youBot two-arm cooperative robot and allowed control of its joints. Thus, the movement of the user’s arm, resulted by muscle contraction, is transposed into joints movement of the robotic arm. At the end of the paper are presented the conclusions of the experiments performed, the utility, but also the directions of future development for the proposed concept.
Jing Ma, Qi-Zhi Zhong, Xiao-Dong Sun et al.
Environmental Science: Nano · 2023
Methanogens are powerful catalysts employed in electrobiological systems that are expected to contribute toward carbon dioxide emissions reduction and energy production. However, the oxygen generated within electrobiological systems can severely...
S. Gerasimova, A. Beltyukova, Anastasia Fedulina et al.
Sensors · 2023
We present a novel closed-loop system designed to integrate biological and artificial neurons of the oscillatory type into a unified circuit. The system comprises an electronic circuit based on the FitzHugh-Nagumo model, which provides stimulation to living neurons in acute hippocampal mouse brain slices. The local field potentials generated by the living neurons trigger a transition in the FitzHugh–Nagumo circuit from an excitable state to an oscillatory mode, and in turn, the spikes produced by the electronic circuit synchronize with the living-neuron spikes. The key advantage of this hybrid electrobiological autogenerator lies in its capability to control biological neuron signals, which holds significant promise for diverse neuromorphic applications.
A. Krawczyk, T. Skoczkowski
IEEE Transactions on Magnetics · 1996
The paper shows how biological structures (membranes) are modelled when subjected to electromagnetic field. A short explanation of what such an interaction is for is given. The electromagnetic features of a biological cell are given and then, on the basis of it, the mathematical model is presented. The numerical approach is shown and some relevant conclusions are quoted.
M. Krzemieniewski, J. Rodziewicz
Environmental Engineering Science · 2005
The goal of this work was to determine the impact of electric current on the efficiency of removal of nitrogen compounds from waste water. The experiments were run at bench scale, in a rotating ele...
Joanna Rodziewicz, Artur Mielcarek, Wojciech Janczukowicz et al.
Energies · 2023
Due to the high nitrogen and phosphorus concentrations in wastewater from soilless cultivation of tomatoes, the sludge formed during wastewater treatment can be used as a source of nutrients in agriculture. The effect of electrical direct current (DC) density (J) and hydraulic retention time (HRT) in a rotating electrobiological contactor (REBDC) on the quantity and quality of sludge generated in the process of tomato soilless cultivation wastewater treatment was determined. The cathode consisted of discs immobilized with biomass, while the anode was an aluminum electrode. HRTs of 4 h, 8 h, 12 h, 24 h and (J) of 0.63, 1.25, 2.50, 5.00, and 10.00 A/m2 were applied. The study showed that the increase in (J) caused an increase in the amount of sludge generated. The increase of (J) contributed to increased concentrations of biogenes in the sludge, whereas extension of HRT at a given current density contributed to increased concentrations of nitrogen and phosphorus (up to 6.2% and 0.8% respectively) and to the reduced content of organic matter (up to 23.04%). The examined sludge was stabilized, sanitary, and safe, and may be an alternative to mineral fertilizers. This study showed that larger sludge amounts are produced in a DC mode aerobic REBDC than in an alternating current anaerobic one.
Joanna Rodziewicz, Artur Mielcarek, Wojciech Janczukowicz et al.
Water · 2020
The study was conducted in a one-stage rotating electrobiological disk contactor (REBDC) and a rotating electrochemical disk contactor (RECDC). Synthetic wastewater with characteristics similar to the wastewater from soil-less cultivation of tomatoes was used in the experiment. Current efficiency (CE) values, that express the denitrification performance of bio- and electrochemical reactors, were higher in the electrobiological contactor than in the electrochemical one. Combining biological processes with electrochemical processes in the electrobiological contactor resulted in almost 20% higher current efficiency in the contactor operated at a density of 0.63 A/m2 and hydraulic retention time (HRT) = 4 h. The study showed that, in both the electrochemical and the electrobiological contactor, current density increase and hydraulic retention time extension increased electric power consumption (E) during phosphorus compounds removal and simultaneously lowered current efficiency.
I N Deryabina
Ekologiya cheloveka (Human Ecology) · 2016
The paper shows the study results of a brain electrobiological activity in elderly women with various level of cognitive impairment. Importance of early diagnostic of cognitive disorders and applicability of methods of functional brain imaging in revealing disorders in higher cortical functions are shown in the paper. To assess cognitive functions we applied express-method of evaluating of cognitive functions during normal aging. According to the test results three groups were formed: 1 - women without cognitive disorders, 2 -with mild cognitive impairment, and 3 - with moderate cognitive impairment. The EEG was registered for all participants by means of 128-channel system GES-300. Absolute spectral power for all bands was calculated. According to the spectral analysis it has been revealed that higher power of the slow-wave rhythms is characteristic of groups with disorders of cognitive functions. Prevalence of a delta rhythm in the general EEG has been also stated. These changes are perhaps caused by dysfunction of the activating cholinergic systems and cortical structures, and also both dyscirculatory and structural changes of brain matter.
Andrzej Krawczyk, Tadeusz Skoczkowski
COMPEL - The international journal for computation and mathematics in electrical and electronic engineering · 1994
The interactions of electromagnetic fields and biological structures are of great interest both from medical and technological points of view. The medical aspect is well‐known even in archaic medicine while bio‐electrotechnology is being developed just at recent time. Especially it is readily seen when dealing with electric field and its influence on living cells. Biologists and biochemists are mainly interested in the voltage induced in the cell membrane ( transmembrane voltage ). This gives the information about forces acting on membrane which cause the phenomenon called dielectrophoresis . The other phenomenon which joins electromagnetic field and biological structures is electroporesity when the pores in a membrane are caused by electric field. It seems that the latter case requires carefully carried calculations since the transmembrane voltage decides on the phenomenon. The paper, however, does not aim at very biological effect; our goal is to show how the electric field in the cell and the membrane voltage should be calculated. It should be stressed that the research in bio‐electromagnetism is on very initial step as concerns the calculation methods. This results from two facts: the first is that so far there were no links between people working on computational electromagnetism and biologists and the second comes from the complex nature of biological structures which cannot be modelled as easy as it is with technical products. Thus, the paper is aiming at showing how the cell is modelled and what the main dependencies which govern electromagnetic phenomena are. This may be considered as an introductory step to further activity in this area.
Vera N. Gledacheva, Iliyana D. Stefanova, Valeri I. Slavchev et al.
Folia Medica · 2020
Introduction: Examination of the potential possibilities of 2-chloro-N-(1-(3,4-dimethoxyphenyl)propan-2-yl)-2-phenylacetamide (IQP) to affect bioelectrogenesis and the contractile activity of isolated smooth muscles (SM) from stomach. Aim: Having in mind the structural similarities between the molecules of papaverine and IQP, the aim of the present study was to examine such features of the newly synthesized molecule that may potentially affect the muscle tonus, spontaneous bioelectrical and contractile activities of smooth muscles isolated from the stomach, basing on specific mechanisms of papaverine. Materials and methods: The synthesis of IQP is based on the initially formed aziridine ring by principles of Gilbert&rsquo;s reaction. Impact of IQP on the bioelectrogenesis and the contractile activity of isolated smooth muscles from male Wistar rats was measured by the single sucrose-gap method and isometrically recorded. Results: IQP (1&times;10-5 &ndash; 2.5&times;10-4 mol/l) causes muscle relaxation, producing changes in two processes that have influence on the mechanical activity of smooth muscles:1.&nbsp;&nbsp; &nbsp;Blocked Ca2+ influx through the potential-dependent membrane Ca2+ channels, followed in turn by lowering the Ca2+ intracellular levels. This effect is proved by the changes in the frequency and amplitude of spike-potentials in sucrose-bridge experiments when IQP is applied.2.&nbsp;&nbsp; &nbsp;Activation of a cAMP-dependent signal cascade. The relaxing effect of IQP was significantly reduced in the presence of KT5720(5&times;10-6 mol/l), an inhibitor of protein kinase A. Conclusion: We assume that there might be interconnections between these two IQP-dependent processes, because PKA-dependent phosphorylation of the L-type Ca2+ channels in smooth muscles provokes a reaction of inactivation.