Sequence-to-Function Prediction

Gene mapping with DNA gradient visualization and functional analysis - Dr. Shayan Sayyadi

Sequence-to-Function Prediction

Gene mapping with DNA gradient visualization and functional analysis

DNA Sequence Profile: Cytochrome c oxidase

146092113811842 bp

Sequence Length

1,842 bp

GC Content

58%

Coding Density

95%

Host System

E. coli BL21

Sequence Type

Sequence Length (bp)1,842 bp

GC Content (%)58% GC

Codon Optimization (%)85%

Promoter Strength

Plasmid Copy Number20 copies

Expression System

Induction Level (fold)4.5x

Protein Expression

2.01

relative units

Activity

76.0%

enzymatic activity

Folding Probability

95.0%

properly folded

Stability

21.1

hours half-life

Predicted Functional Domains

Heme-binding domain

Signal peptide

DNA-binding domain

Sequence Metrics

CAI Score:0.710

Cellular Location:Periplasm

Metabolic Burden:60.1%

Electron Transfer:1.83x WT

Predicted Regulatory Elements

Ribosome binding site

Promoter -10 box

Promoter -35 box

CAP binding site

Sequence Optimization Recommendations

Outstanding electron transfer capability - ideal for bioelectrochemical applications.